炙甘草汤对气血两虚证心律失常大鼠血清炎症因子和心肌酶的影响_药学论文.docVIP

炙甘草汤对气血两虚证心律失常大鼠血清炎症因子和心肌酶的影响_药学论文 炙甘草汤对气血两虚证心律失常大鼠血清炎症因子和心肌酶的影响_药学论文 作者：陈兰英，罗雄，罗颖颖，胡瑞刚，龚琴，陈筱，陈奇 【摘要】 目的研究炙甘草汤对气血两虚证心律失常大鼠的保护作用。方法在放血和环磷酰胺并用致使大鼠气血两虚的基础上舌下静脉注射适量乌头碱，复制大鼠气血两虚证心律失常模型，并用炙甘草汤进行防治，观察炙甘草汤对模型大鼠心电图，血清心肌酶及血清炎症因子超敏C反应蛋白(hs-CRP)、肿瘤坏死因子α(TNF-α)、白介素-2(IL-2)、白介素-6(IL-6)水平的影响。 结果炙甘草汤能明显改善模型大鼠心电图、延长心律失常的潜伏期及缩短心律失常的维持期;明显升高模型大鼠的hs-CRP、TNF-α、IL-2以及IL-6水平，显著降低心肌酶CK和LDH的水平。结论炙甘草汤对气血两虚证心律失常大鼠的保护作用可能与改善介导心肌细胞的炎症反应及心肌酶的活性有关。 【关键词】 炙甘草汤; 心律失常; 气血两虚; 血清炎症因子; 心肌酶 　　Abstract：ObjectiveTo study the protective action of Zhigancao Decoction on arrhythmia with deficiency of both Qi and blood in rats.MethodsOn the basis of syndrome of both Qi deficiency and blood deficiency in rats induced by bloodletting and cyclophosphamide, arrhythmia was induced by aconitine, and Zhigancao Decoction was used to prevent and treat arrhythmia rats with syndrome of deficiency of both Qi and blood.The changes of ECG were observed, and activities of hs-CRP、TNF-a、IL-2,IL-6 ,CK and LDH were determined. Results Zhigancao Decoction could improve the ECG, increase arrhythmia latency and decrease arrhythmia duration significantly. At the same time, Zhigancao Decoction could obviously improve the levels of hs-CRP, TNF-α, IL-2 and IL-6 in model rats, and markedly reduce the levels of CK and LDH in model rats. ConclusionThe protective action of Zhigancao Decoction on arrhythmia with deficiency of both Qi and Blood in rats may be related to the improvement of the medicator released by inflammatory response and the enzyme of myocardium. 　　Key words：Zhigancao Decoction; Arrhythmia; Deficiency of both Qi and blood; Inflammatory factor; Myocardial enzyme 　　炙甘草汤出自东汉张仲景的《伤寒论·辨太阳病脉证并治》篇第177条，“伤寒，脉结代，心动悸，炙甘草汤主之”，是中医治疗心悸胸痛的经典名方。在中医临床上，很多心律失常患者也往往伴有气血亏虚的症状，即气血两虚心律失常，炙甘草汤治疗气血两虚心律失常是在补益气血的基础上发挥复脉效果。为进一步探讨炙甘草汤的作用机理，采用贴近中医症候的病证结合模型复制大鼠气血两虚证心律失常模型，观察炙甘草汤对气血两虚证心律失常大鼠血清hs-CRP、TNF-α、IL-2、IL-6水平以及心肌酶的影响，从而分析该方对气血两虚心律失常的保护作用。 　　1 材料与仪器 　　1.1 药物炙甘草汤原药材购于江西中医学院附属医院中药房，经江西中医学院中药鉴定教研室鉴定均符合2005版《中国药典》标准，将购得的炙甘草汤原药材加入适量的冷蒸馏水浸泡30 min，然后