热休克蛋白70对兔未成熟心肌和心肌间质的影响_医学论文.docVIP

热休克蛋白70对兔未成熟心肌和心肌间质的影响_医学论文 热休克蛋白70对兔未成熟心肌和心肌间质的影响_医学论文 作者：孙忠东，宋玉娥，池一凡，夏家红，杨铁南，肖诗亮，杨辰垣 【关键词】 心肌间质 Effects of heat shock protein 70 on immature myocardium and myocardial interstitium in rabbits 　　【Abstract】 AIM: To investigate the protective effects of heat shock protein 70 (HSP70) on immature myocardium and myocardial interstitium. METHODS: Isolated working rabbit heart model was used in this study. Twelve rabbits (aged 14-21 days) were randomly divided into 2 groups: Control group (C) undergoing 45 min ischemia followed by 45 min reperfusion after distilled water was injected by intraperitoneum 24 h later, and experimental group (E) undergoing 45 min ischemia followed by 45 min reperfusion after norepinephrine was injected by intraperitoneum 24 h later. The HSP70 content, lactate dehydrogenase (LDH) and creatine kinase (CK) leakage, adenosine triphosphate (ATP) and malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, hydroxyproline (HP) and endothelin (ET) content, myocardial cell Ca2+ content, Ca2+ATPase activity of mitothondia (［Ca2+ATPase］m) and its Ca2+ content (［Ca2+］m), synthesizing ATP activity of mitochondria(［ATP］m) and myocardial ultrastructure were tested. RESULTS: The cardiac function recovery was better in E group than C group (0.05). The HSP70 content, ATP content, SOD activity, HP, ［Ca2+ATPase］m activity and ［ATP］m activity were higher in E group than C group (0.01). The MWC, LDH and CK leakage, MDA content, ET, myocardial cell Ca2+ content and ［Ca2+］m content were lower in E group than C group (0.01). The myocardial ultrastructure injuries were milder in E group than in C group. CONCLUSION: HSP70 has obvious protective effects on immature myocardium and myocardial interstitium. 　　【Keywords】 heat shock protein 70 immature myocardium myocardial interstitium cardioprotection 　　【摘要】 目的: 探讨热休克蛋白70(HSP70)对未成熟心肌和心肌间质的影响. 方法：健康新生长耳大白兔12只随机分为2组. 对照组(C组)：ip生理盐水0.4 mL，24 h后取离体心脏，常规建立Langendorff离体心脏灌注模型，灌注15 min转为工作心15 min后停灌45 min，恢复灌注15 min改为工