甲基东莨菪碱和吡啶斯的明对鼠应激性体温过高反应的影响_医学论文.docVIP

甲基东莨菪碱和吡啶斯的明对鼠应激性体温过高反应的影响_医学论文 甲基东莨菪碱和吡啶斯的明对鼠应激性体温过高反应的影响_医学论文 作者：杨永录　赖雁　何彦芳　樊萌　李欣 【摘要】 　　目的 外周胆碱能神经参与细菌内毒素或者内生致热原引起的发热，本实验的目的是探讨是否外周胆碱能神经对应激性发热也有类似的作用。方法 用无线遥测方法观察皮下注射甲基东莨菪碱和吡啶斯的明对大鼠在开放环境中应激性体温过高和活动的影响。用分光光度技术测定血浆胆碱酯酶的活性。结果 外周胆碱能受体阻断剂甲基东莨菪碱可阻断暴露在开放环境中大鼠的体温过高反应，与盐水组大鼠比较体温升高明显减弱。给大鼠吡啶斯的明可引起血浆胆碱酯酶的活性降低52%，而导致暴露在开放环境中大鼠的体温过高反应明显提高。给动物注射甲基东莨菪碱后，立即给吡啶斯的明，几乎可以抵消甲基东莨菪碱对开放应激体温反应的影响。但是，这两种药物对暴露在开放环境大鼠的活动没有影响。结论 实验结果表明，吡啶斯的明和甲基东莨菪碱可以影响暴露在开放环境中大鼠的体温调节反应，提示外周胆碱能神经参与大鼠应激性体温过高反应的调节。 【关键词】 体温；应激性体温过高；外周胆碱能神经 Abstract：Objective It has been reported that peripheral cholinergic nerve may be involved in the fever induced by bacterial endotoxin or endogenous pyrogen.The aim of present experiment was to investigate whether peripheral cholinergic nerve has also similar effect on stress-induced hyperthermia.Methods Effects of subcutaneous injection of methyl scopolamine and pyridostigmine on stress-induced hyperthermia and motor activity were observed in rats by radio telemetry in an open-field chamber. Plasma cholinesterase(ChE)activity was measured by a spectrophotometry.Results Methyl scopolamine,a peripheral muscarinic antagonist,leaded to a significant attenuation in the hyperthermic response to open-field exposure compared with rats dosed with saline.The rat with pyridostigmine that caused a 52% decrease in plasma ChE activity led to a significant enhancement of the hyperthermic response to open-field exposure.Pyridostigmine coadministered with methyl scopolamine nearly abolished the effects of methyl scopolamine on core temperature during open-field stress.But the rats treated with pyridostigmine and methyl scopolamine were not effected significantly on motor activity to open-field exposure compared with rats dosed with saline.Conclusions Experimental data showed that methyl scopolamine and pyridostigmine could interfere with the thermoregulatory response to open field exposure,and indicated that peripheral cholinergic nerve involved in the control of stress hyperthermic respons