甜荞麦叶总黄酮降糖、降脂作用及机制_药学论文.docVIP

甜荞麦叶总黄酮降糖、降脂作用及机制_药学论文 甜荞麦叶总黄酮降糖、降脂作用及机制_药学论文 作者：刘淑梅 韩淑英 崔国金 朱丽莎 吕华 【关键词】 荞麦叶总黄酮 　　Effects and mechanism of total flavones of buckwheat leaf in lowering serum glucose and lipid 　　【Abstract】 AIM: To study the effects and mechanism of total flavones of buckwheat leaf (TFBL) in lowering serum glucose, lipid and antilipid peroxidation in diabetic and hyperlipidemia rats. METHODS: The diabetic and hyperlipid model of rats were established by streptozotocin and high lipid feed. The levels of serum glucose, TC, TG, insulin, MDA, SOD and insulin receptor of liver were detected after TFBL was administered orally with the dose of 0.2, 0.4 and 0.8 g#12539;kg-1 for 12 wk. RESULTS: TFBL obviously decreased the serum glucose, TG and LDLc (0.01), increased HDLc (0.01), enhanced both the insulin sensitivity index and the combination of insulin with receptor, and improved the glucose tolerance. TFBL also obviously lowered the content of MDA (0.01) and improved the SOD activity in blood. CONCLUSION: TFBL has the effects of reducing serum glucose, lipid, MDA, antilipid peroxidation and increasing the insulin receptor quantity and/or sensitivity. 　　【Keywords】 total flavones of buckwheat leaf; diabetic; hyperlipidemia; serum glucose; serum lipid; lipid peroxides 　　【摘要】 目的: 研究荞麦叶总黄酮（TFBL）对实验性糖尿病高脂血症大鼠的降血糖、血脂作用及其机制. 方法: 用链脲佐菌素和高脂饲料诱发大鼠糖尿病和高脂血症，以TFBL口服治疗12 wk，剂量分别为0.2, 0.4和0.8 g#12539;kg-1. 实验结束时检测空腹血糖（FBG）、糖耐量(OGTT), TC, TG, LDLC, HDLC, 胰岛素（INS）、血清和肝组织脂质过氧化产物丙二醛（MDA）、超氧化物歧化酶(SOD)及肝细胞膜胰岛素受体结合力. 结果: TFBL能降低血中FBG， TG， LDLC（0.01），升高HDLC（0.01），改善OGTT，增加胰岛素敏感指数（ISI）和胰岛素与受体的结合力，存在剂量依赖性. 同时TFBL能降低血清和肝组织中MDA含量（0.01），增加血清SOD活力. 结论: TFBL具有良好的降血糖、调血脂、增加胰岛素与受体结合力和敏感性及抗脂质过氧化作用. 　　【关键词】 荞麦叶总黄酮； 糖尿病； 高脂血症；血糖；血脂；脂质过氧化 　　0引言 　　糖尿病（DM）高脂血症的发病率呈逐年上升趋势并严重危害人类健康. 因此，研究和开发防治DM及并发症的药物越来越受到人们重视，特别是从中草药中寻找有效防治糖尿病高脂血症及并发症的药物倍受人们关注. 荞麦为蓼科本草植物, 其全草含较高的黄酮类化合物. 流行病学研究表明荞麦产区人群血糖、血脂水平低于非荞麦产区. 国外学者研究发现荞麦种子提取物具有降糖、降脂、抗氧化及抑制血小板聚集等活性［1,2］. 荞麦叶总黄酮(total flavones of buchwheat leaf, TFBL)主要含有芦丁、槲皮素，但荞麦叶总黄酮和纯度明显高于种子. 目前对荞麦种子研究报道