生存素和nm23_药学论文.docVIP

生存素和nm23_药学论文 生存素和nm23_药学论文 【关键词】 生存素 　　摘要：目的探讨肝细胞肝癌（HCC）中生存素（Survivin.）和nm23H1表达的临床意义及两者的相互关系。方法采用免疫组织化学SP法检测40例HCC及26例癌旁肝组织中Survivin 和nm23H1的表达。结果①Survivin、nm23H1在HCC中的阳性表达率分别为57.5%（23/40）、42.5%（17/40），两者阳性表达率明显分别高于、低于癌旁肝组织（P＜0.01）；②Survivin的表达与HCC的分化程度、转移、癌栓有关（P＜0.05）,而与HCC患者的性别、发病年龄、原发肿块大小无关（P＞0.05）；③nm23H1的表达与HCC的转移、癌栓有关（P＜0.05）,而与HCC患者的性别、发病年龄、原发肿块大小及分化程度无关（P＞0.05）；④在HCC中，Survivin与nm23H1间的表达无明显相关性（r=0.125,P＞0.05）。结论 Suvivin和nm23H1可作为反映HCC发生发展及预后判断的重要指标。 　　关键词：癌；肝细胞；生存素；nm23H1；免疫组织化学 　　Expression of Survivin and Nm23H1 and their Clinical Significance in Hepatocellular Carcinoma 　　Abstract：ObjectiveTo investigate the expression and the clinical significance of survivin and nm23H1 and their correlations in hepatocellular carcinoma(HCC).Methods Expressoin of Survivin and nm23H1 in 40 HCC samples and in 26 samples from neighboring regions of correlative HCC were detected by using immunohistochemical SP method. Results①Survivin and nm23H1 expression rate were detected at 57.5%（23/40）and 42.5%（17/40）respectively. The positive expression rate of survivin and nm23H1 in HCC were significantly higher and lower than in their neighboring regions(P＜0.01). ②The expression of survivin in HCC had association with the differentiated level、the metastasis and the cancer embolus (P＜0.05), but no association with the patient sexuality、age、and the tumor size (P＞0.05).③ The expression of nm23H1 in HCC had association with the metastasis and the cancer embolus(P＜0.05),but no association with the patient sexuality、age、the tumor size and the differentiated level (P＞0.05). ④ In the cases of HCC, there was no significant correlation between the expression of survivin and nm23H1.ConclusionExpression of survivin and nm23H1 might be important markers in reflecting of carcinogenesis、progression and prognosis of HCC. 　　Key words：Carcinoma, Hepatocellular; Survivin; Nm23H1; Immunohistochemistry 　　原发性肝细胞肝癌（Hepatocellular carcinoma. HCC）是常见的恶性肿瘤之一，统计学资料表明［1］： HCC已上升为我国恶性肿瘤的第二位，且发病年龄有年轻化趋势，目前以手