电感耦合等离子体质谱分析砂仁中微量元素的溶出特性及形态_药学论文.docVIP

电感耦合等离子体质谱分析砂仁中微量元素的溶出特性及形态_药学论文 电感耦合等离子体质谱分析砂仁中微量元素的溶出特性及形态_药学论文 作者：罗盛旭，李金英，胡广林，贾振亚 【摘要】 目的分析砂仁中微量元素的溶出特性及形态，探讨微量元素的生物活性。方法采用电感耦合等离子体质谱（ICP-MS）测定砂仁中13种元素含量及溶出率，分别用0.45 μm滤膜，正辛醇-水萃取体系，将砂仁水煎液中元素区分为可溶态与悬浮态、醇溶态与水溶态，并分析各形态。结果所建立的分析方法，标准曲线的相关系数为0.999 8～1.000 0, RSD为0.1%～5.0%，测定标准物质灌木枝叶中元素含量与参考值有良好的一致性。砂仁中Co溶出率最高，各元素溶出率并非按照元素在药材中总量高低排序。砂仁的元素溶出以悬浮态居多，仅Cr的可溶态/总溶出大于50%。水煎液中 Fe、Mo和Cd的Kow参数在胃、肠液酸度下均大于1，而Cr、 Ni的Kow参数均小于1，元素的Kow值受pH影响。结论砂仁中微量元素的溶出特性及形态，与砂仁的生物活性与功效具有一定关系。 【关键词】 砂仁； 微量元素； 溶出特性； 形态分析； 电感耦合等离子体质谱 　　Abstract：ObjectiveTo analyze the dissolution characteristics and speciation of trace elements in Amomum villosum and investigate the bioactivity of trace elements. MethodsThe content and dissolved rate of 13 trace elements in Amomum villosum were analyzed by ICP-MS with 0.45 μm membrane , the n-C8H17OH -water extraction system respectively.Divided the decoction of Amomum villosum into several species, including the soluble species, suspended species or the n-C8H17OH-soluble species and water-soluble species, and studied every species. ResultsThe correlative coefficients of the analytical calibration curves were 0.999 8～1.000 0,and RSD was 0.1%～5.0%. The accuracy and precision of the method were tested by comparing the values obtained from the determination of the standard sample, bush twigs and leaves（GBW07603）by this method with the reference values of GBW07603. The determination results were found to be basically consistent with the reference values.The highest dissolved rate was Co in the decoction of Amomum villosum, the dissolved rate of element did not depend on the content of trace element in the drug. The trace elements of Amomum villosum mainly existed in suspended species. Only Cr,its content in soluble species was more than 50% of its whole content in all species, and the Kow values of Fe, Mo, and Cd were all more than 1 under the pH of intestinal and stomach juice,while Cr, Ni were less than 1. Kow values of trace elements was influenced by different pH value