白藜芦醇体外抑制人胰腺癌细胞增殖的实验研究_医学论文.docVIP

白藜芦醇体外抑制人胰腺癌细胞增殖的实验研究_医学论文 白藜芦醇体外抑制人胰腺癌细胞增殖的实验研究_医学论文 【摘要】 目的:探讨白藜芦醇(resveratrol，Res)体外对胰腺癌MIAPaCa-2细胞增殖的影响，并探讨其初步机制。方法:采用四甲基氮唑蓝法（MTT）检测Res处理后MIAPaCa-2细胞的增殖情况。用倒置显微镜、透射电镜观察Res处理后细胞形态学改变，用流式细胞仪分析经25μmol·L-1、50μmol·L-1、100μmol·L-1、200μmol·L-1 Res分别作用48 h后MIAPaCa-2细胞凋亡率。结果:Res体外能明显抑制MIAPaCa-2细胞的生长增殖，且在一定范围内呈时间、浓度依赖性。经Res处理后，倒置显微镜观察到细胞数目减少，部分细胞变圆，核浓缩，提示可能存在细胞凋亡；电镜证实存在典型的细胞凋亡形态学改变。用25μmol·L-1、50μmol·L-1、100μmol·L-1、200μmol·L-1 Res处理48 h后，流式细胞仪检测出各处理组的细胞凋亡率明显高于对照组(0.05)。结论:Res在体外能明显抑制MIAPaCa-2细胞的增殖，诱导细胞调亡可能是Res抑制MIAPaCa-2细胞增殖的主要机制之一。 【关键词】 白藜芦醇 胰腺癌 增殖 凋亡 Abstract: Objective:To explore the effect of resveratrol(Res) on the proliferation of pancreatic carcinoma MIAPaCa-2 cells in vitro and explore the rudimental mechanism. Methods: Methyl thiazolyl tetrazolium method (MTT) was used to detect the cell growth status of MIAPaCa-2 cells after treatment with Res. The morphologic changes of cells after treatment with Res were detected by inverted microscope and transmission electron microscope. The apoptosis rates were analyzed with flowcytometry(FCM) after the cells were treated with 25μmol·L-1, 50μmol·L-1, 100μmol·L-1 and 200μmol·L-1 Res for 48 h, respectively. Results: Res inhibited the proliferation of MIAPaCa-2 cells in a concentration-and time-dependent manner as measured by MTT method. After the cells were treated with Res, inverted microscope observed the reduction of the cell number and found significant morphologic changes, characterized by cell rounding and cell nuclear enrichment suggesting apoptosis. And the presence of typical morphological changes of apoptosis was confirmed with transmission electron microscope. The apoptosis rates at 25μmol·L-1, 50μmol·L-1, 100μmol·L-1 and 200μmol·L-1 Res-treated groups after treatment for 48 h, measured by FCM, were significantly higher than those in control groups(0.05). Conclusion: These findings suggest that the natural product Res may have a potent anti-proliferative effect on MIAPaCa-2 cells,the mechanisms of which may be