磁共振灌注成像评价兔后肢缺血模型肌肉灌注的实验研究_医学论文.docVIP

磁共振灌注成像评价兔后肢缺血模型肌肉灌注的实验研究_医学论文 磁共振灌注成像评价兔后肢缺血模型肌肉灌注的实验研究_医学论文 作者：孟利民 张挽时 宋云龙 吴冰 王东 毕永民 祝红线 【关键词】 磁共振成像；灌注；缺血；疾病模型,动物 　　【Abstract】 AIM：To evaluate the application of magnetic resonance (MR) perfusion weighted imaging in the assessment of muscle perfusion in hindlimb ischemia model of rabbit. METHODS: Ischemia was induced in right hind limbs of 16 New Zealand white rabbits by complete excision of femoral artery. Before and 1, 3, 7, 14, 21, 28, 42, 56, 70 d after the model establishment, MR perfusion scan was performed on models respectively and the percent MR signal enhancement of the region of interest was plotted to draw a timeenhancement curve. The perfusion curve and parameters (enhancement onset time, peak value and slope rate) were analyzed. RESULTS: Following femoral artery excision, a rapid reduction of muscle perfusion in right hindlimb was observed. Perfusion recovered very slowly during the first 7 d postexcision and faster perfusion recovery was observed within 14 to 70 d, but at last perfusion didnt reach the normal level. CONCLUSION: MR perfusion imaging is a safe, noninvasive, semiquantitative and relatively accurate technique to assess timedependent changes of muscle perfusion in hindlimb ischemia model of rabbit. 　　【Keywords】 magnetic resonance imaging perfusion ischemia disease models, animal 　　【摘要】 目的：初步探讨磁共振灌注成像（MRPWI）在评估兔后肢缺血模型肌肉组织灌注中的应用价值. 方法：对16只新西兰大白兔建立右后肢缺血模型，分别于术前、术后1, 3, 7, 14, 21, 28, 42, 56, 70 d行MR灌注成像检查，原始灌注图像资料经Mean Curve软件处理，将感兴趣区（ROI）信号强度变化绘制成时间信号增强百分比曲线，分析该灌注曲线特征并观察灌注参数（注药后开始强化时间、强化峰值、强化斜率）变化趋势. 结果：术后右后肢血流灌注较对侧急剧减低，术后7 d内灌注恢复非常缓慢，从14 d起灌注恢复逐渐加快，但直至术后70 d仍未恢复至正常灌注水平. 结论：磁共振灌注成像能够安全、无创、半定量地且较准确地评估动物后肢缺血模型随时间推移发生的肌肉组织灌注变化. 　　【关键词】 磁共振成像；灌注；缺血；疾病模型，动物 　　0引言 　　磁共振灌注成像（magnetic resonance perfusion weighted imaging, MRPWI）是通过观察水溶性对比剂在组织中的早期分布特点来了解组织血管化程度、血流灌注状况、毛细血管通透性等生理信息的功能成像方法［1-2］. 目前，MR灌注加权成像在脑缺血、脑肿瘤等病变的血流灌注研究中应用较广，对缺血性肢体疾病的研究尚在初步探索中. 本文旨在通过建立兔后肢缺血模型，探讨MRPWI在评估缺血肌肉组织灌注状态中的应用价值. 　　1材料和方法 　　1.1材料由空军总医院动物实验中心提供纯种新西兰大白兔16只，雌雄不限，体质量2.3～3.