实验性自身免疫性脑脊髓炎的小胶质细胞和星形胶质细胞反应.docVIP

实验性自身免疫性脑脊髓炎的小胶质细胞和星形胶质细胞反应 文章来源：有问必答健康社区 2006/7/24 10:12:36 文字大小：【大】【中】【小】实验性自身免疫性脑脊髓炎的小胶质细胞和星形胶质细胞反应 中华神经科杂志 1999年第2期第32卷 论著 作者：于国平　朱克　梁显胤　田东华　周湘军 单位：100853 北京，解放军总医院神经内科(于国平、朱克、田东华、周湘军)；吉林省通化市中心医院神经内科(梁显胤) 　　关键词： 脑脊髓炎；小神经胶质细胞；星形细胞；免疫组织化学 　　【摘要】　目的　确定小胶质细胞和星形胶质细胞在实验性自身免疫性脑脊髓炎(EAE)发病及病变发展中的作用。方法　用牛脊髓髓鞘碱性蛋白（MBP）免疫豚鼠发生EAE，用免疫组化法观察EAE不同病期小胶质细胞和星形胶质细胞对炎性脱髓鞘病灶的反应。结果　发生EAE的前3天，小胶质细胞即开始激活，在临床症状出现时其数量及激活程度达高峰，并持续至高峰期。恢复期数量逐渐减少，激活程度逐渐减弱。星形胶质细胞在症状高峰期开始激活并围绕在浸润细胞和病变血管周围，似有隔离小胶质细胞与病灶接触的作用，至恢复期激活明显。结论　小胶质细胞激活在EAE的发病及进展中起重要作用，而星形胶质细胞主要与疾病的恢复有关。 　　Effects of microglia and astrocytes on inflammatory lesions of the central nervous system in experimental autoimmune encephalomyelitis　YU Guoping, ZHU Ke, LIANG Xianyin, et al. 100853 100853 　　【Absteact】　Objective　To explore the role of microglia and astrocytes in the pathogenesis and lesion progress in experimental autoimmune encephalomyelitis (EAE).Methods　We induced EAE in guinea pigs using bovine MBP and examined microglial and astroglial reactions to EAE lesions at various stages with the method of immunohistochemistry. For the demonstration of the microglia and astrocytes, the antibodies against RCA-1 and glial fibrillary acidic protein (GFAP) were used.Results　The microglia began to become active three days before the appearance of clinical symptoms. At the initial stage of EAE, when the symptoms manifested themselves evidently the microglia were increased in number and in activity reaching their peaks in close association with the development of the lesions, whereas the astrocytes showed no significant reaction. At the peak stage of EAE, the astrocytes began to be active so that their fibers enclosed the inflammatory lesions. At the recovery stage of EAE, the astrocytes became significantly active, whereas the microglia reaction became gradually inactive and the microglia got slowly less and less in number.Conclusion　It is suggested that the the microglia augment, but the astrocytes suppress, the inflammatory processes in the