尾加压素Ⅱ 与中介素在牛磺酸减轻油酸所致大鼠肺损伤中的作用.docVIP

尾加压素Ⅱ与中介素在牛磺酸减轻油酸所致大鼠肺损伤中的作用 宋 斌1,2,于忠和3,郑 涛2（400038 重庆，第三军医大学研究生管理大队1；100700 北京 北京军区总医院：重症监护科2,全军肿瘤内科？诊治中心3） [摘要] 目的 研究牛磺酸对油酸所致急性肺损伤大鼠的干预作用及其可能的作用机制。通过影响尾加压素Ⅱ（UⅡ）及中介素（intermedin，IMD）合成与分泌发挥肺保护作用的机制。方法 采用油酸性急性肺损伤大鼠模型。动物分为3组，对照组（C组）、油酸损伤组（A组）、牛磺酸治疗组（T组），分别于0、6、12、24 h 4个时间抽动脉血2 mL并取肺泡灌洗液（BALF），测动脉血氧分压[p(O2)]，血浆及BALF中UⅡ、IMD浓度，测定肺湿干质量比（W/D）并进行统计学分析；HE染色、免疫组化检查肺组织中UⅡ与IMD表达水平；电镜观察肺组织超微结构。结果 T组p(O2)在6、12、24 h时高于A组（P＜0.05）；A组肺组织W/D在0 h和6 h高于T组及C组（P＜0.05）；A组血浆UⅡ在24h高于C组及T组（P＜0.05）；3组BALF UⅡ在0、6 h差异显著（P＜0.05），0 h时T组高于C组及A组（P＜0.05），6 h时A组高于T组（P＜0.05）；A组血浆IMD在0 h时高于T组及C组（P＜0.05）。光镜观察：T组肺组织损伤减轻。免疫组化染色结果显示各组大鼠支气管黏膜上皮细胞、平滑肌细胞，肺内血管内皮细胞、平滑肌细胞细胞膜及细胞质均有UⅡ与IMD阳性表达，A组与T组间表达无明显差异。电镜观察： T组较A组损伤减轻，基底膜完整，未见明显胶原增生。结论 牛磺酸可减轻油酸致大鼠急性肺损伤，其机制可能与抑制UⅡ表达，减轻毛细血管基底膜损伤后肺泡渗出有关。 [关键词] 油酸；急性肺损伤 尾加压素Ⅱ 中介素 牛磺酸 [中图法分类号] [文献标志码] A The Effects of Urotensin-II and Intermedin on Taurine Alleviating Oleic Acid induced Acute Lung Injury of Rats Song Bin 1,2,Yu Zhonghe3,Zheng Tao2 (1, Master of Third Military Medical University ,2 Cancer Treatment Center of Beijing Military General Hospital, Beijing 100700) [Abstract] Objective: To study the effect of taurine on oleic acid induced acute lung injury (ALI) of rats and its possible mechanisms, especially the mechanisms mediated by the synthesis and secretion of urotensin-II (U-II) and intermedin (IMD).Methods: Rat models of acute lung injury induced by oleic acid were used. The animals were divided into three groups: the control group (group C), the oleic acid injury group (group A), and the taurine treatment group (group T). 2ml of blood were drawn from the arteries at 0h, 6h, 12h, and 24h time points while lavage fluid (BALF) was taken to measure arterial oxygen tension (P(O2)), U-II, IMD concentration in plasma and BALF, and lung wet-to-dry ratio (W/D) for statistical analysis; lung specimens were observed by naked eyes, microscopy and immunohistochemical examination to determine the pathological changes in lung tissues; electron micros