塞来昔布对脑胶质瘤细胞放射增敏效应的研究.docVIP

塞来昔布对脑胶质瘤细胞放射增敏效应的研究【摘要】? ［目的］ 观察COX-2选择性抑制剂塞来昔布(Celecoxib)对三种脑胶质瘤细胞放射增敏作用。［方法］ 选择适当浓度的COX-2选择性抑制剂Celecoxib进行放射增敏实验，设置单纯放射（R）组和放射+药物（R+D）组，采用集落形成法分别检测Celecoxib对脑胶质瘤SHG-44细胞株，767细胞以及大鼠脑胶质瘤C6细胞株的放射增敏效应，并绘制细胞存活曲线。［结果］ Celecoxib对3种胶质瘤细胞株均显示出放射增敏效应，R+D组与R组相比较，反映放射敏感性指标的SF2、D0.01、D0、Dq均出现下降。其中tSF2=8.315，P=0.014；tD0.01=9.28，P=0.011；tD0=3.985，P=0.058；tDq=3.348，P=0.075。［结论］ Celecoxib在体外实验中可以提高脑胶质瘤的放射敏感性。Celecoxib有可能成为治疗胶质瘤的放射增敏剂。 【关键词】? 塞来昔布；COX-2；放射敏感性；胶质瘤 ? Radiosensitization of Celecoxib for Glioma Cells ?? QIN Song-bing, ZHOU Le-yuan, XU Xiao-ting, et al. ?? (The First Affiliated Hospital, Suzhou University, Suzhou 215006, China)Abstract: ［Purpose］ To study the radiosensitization of celecoxib to three strains of glioma cells. ［Methods］ Celecoxib with proper concentration was adapted for radiosensitive experiments for SHG-44 cells, 767 cells and C6 cells. The cells were randomized into two groups, radiation alone group (R), and radiation combined with celecoxib group (R+D). Celecoxib’s radiosensitization was measured by clongenic assay, and survival curve was described. ［Result］ Celecoxib displayed radiosensitizing effect in the three strains of glioma cells. The Value of D0, Dq, SF2 and D0.01 of group R+D was significantly lower than that of group R (tSF2=83.5,P=0.014;tD0.01=9.28,P=0.011;tD0=3.985,P=0.058;tDq=3.348,P=0.075). ［Conclusion］ The COX-2 selected inhibitor, celecoxib could enhance radiation effect in three strains of glioma cells in vitro. Celecoxib might become an effected radiosensitizer in the treatment for glioma. ?? Subject words: Celecoxib; COX-2; radiosensitivity; glioma ?? 脑胶质瘤，尤其是高级别恶性胶质瘤，手术难以全切，术后放疗为其重要的治疗手段，但多数脑胶质瘤对射线不敏感，总体治疗效果并不令人满意，预后不佳[1]，复发率较高。其中肿瘤本身的放射敏感性是影响临床疗效的重要因素，因此探究影响胶质瘤放射敏感性的因素并采取有效措施提高其放射敏感性有着重大的临床意义，有可能对恶性脑胶质瘤的治疗带来积极的作用。 ?? 环氧合酶(cyclooxygenase，COX)是花生四烯酸转化为前列腺素的限速酶，存在COX-1和 COX-2两种同工酶，研究表明，COX-2的表达与人类多数肿瘤关系密切，它参与了肿瘤细胞的转化、生长、凋亡过程，同时也参与细胞的运动、转移、肿瘤新生血管的形成[2]。COX-2抑制剂联合化疗药物在对结肠癌、肺癌、乳腺癌的体外和在体研究中取得良好的效果，但对于COX-2抑制剂与放疗联合作用，目前的研究还不多。国外有学者认为CO