龙葵碱对雄性小鼠遗传毒性实验研究.docVIP

龙葵碱对雄性小鼠遗传毒性实验研究 季宇彬1,2王斌1,2 郎朗1,2 （1.哈尔滨商业大学 生命科学与环境科学研究发展中心 药物研究所 博士后科研工作站，黑龙江 哈尔滨150076；2.国家教育部 抗肿瘤天然药物工程研究中心，黑龙江 哈尔滨150076） 摘要 目的 探讨龙葵碱对小鼠骨髓细胞、精子的毒性作用。方法 采用流式细胞仪分析龙葵碱对小鼠骨髓细胞周期的影响；采用微核试验技术观察龙葵碱对小鼠骨髓细胞微核变化的影响；采用精子畸形试验观察雄性小鼠精子畸形率的变化。结果 流式细胞仪检测到龙葵碱对小鼠骨髓细胞周期的结果表明，G0/G1期细胞比率随剂量增加而不断升高,S期和G2/M期比率均有下降。这表明细胞被阻滞在G0/G1期，影响DNA的合成，进而影响G2/M期，使进入这一期细胞数减少,证明龙葵碱对骨髓细胞周期可产生明显的干扰。微核发生率和精子畸形率随剂量增高而逐渐增加。经统计学处理,高中低剂量组与阴性对照组均有统计学意义(P＜0.05)。结论 在本实验条件龙葵碱对雄性小鼠存在遗传毒性影响。 关键词 龙葵碱；细胞周期；骨髓微核；精子畸形 Study of solanin on Genotoxicity of male mice Ji YuBin1,2,WangBin1,2,LangLang1,2 (1. Postdoctoral Research Station, Inistitute of Materia Medica, Life Science and Enviroment Science Research Center, HarBin Commerce University, HeiLongJiang HarBin 150076, china; 2. Engineering Research Center of Natural Anticancer Drugs, Ministry of National Education, HeiLongJiang HarBin 150076, china) [Abstract] Objective:To explore the toxic effect of solanin on myeloid cell and spermatozoon in male mice.Methods: To analyze the effect of solanine on cell cycle of myeloid cell in male mice by flow cytometer(FCM) assay and to study the effect of solanin on myeloid cell and spermatozoon in male mice by micronucleus test of myeloid cell and sperm deformity test. Result: G0/G1 pcriod increase, S and G2/M pcriod decrease in solanine group. It indicates that solanin block myeloid cell in G0/G1 phasc to stop the DNA synthcsis and to reduce cell population of G2/M pcriod.Therefore, Solanin is proved to produce conspicuous interference on myeloid cell. High-dosage groups medium-dosage groups and low-groups of Solanin all show statistical significance(P0.05) compared with antifertility-dosage groups in micronuclei assay of myeloid cell and sperm deformity test. Conclusion:The genetoxic influence of solanin on male mice is existed in such experiment condition. [KeyWords] Solanin; Cell cycle; myeloid cell; sperm deformity 龙葵提取物含多种具有抗肿瘤作用有效药理成分，抗肿瘤作用最主要成分为生物碱，龙葵碱又名茄碱，主要存在于茄科植物马铃薯的块茎及龙葵的全草中。龙葵碱有抗肿瘤作用，对动物肿瘤的抑制率极强,有明显的细胞毒作用[1]。同时龙葵是