人工消化法检验旋毛虫效果及其对肌幼虫的影响.pdfVIP

20 11 23 2 Ch in J Sch isto Contro l 20 11, V o l. 23, N o. 2 211 [ ] 1005666 1( 2011) 0202110 3 人工消化法检验旋毛虫效果及其对肌幼虫的影响 王国英, 都景芳, 顿国庆, 孙伟力, 王金喜 [ ] 100 30 d , 3 , : 2 h () 12 h 20 h, 10, 300 , ; 40 3 ( 21220 h) , 4 , 10 : 100 ; : 3 , 100 30 d , , 21220 h 78. 47% 76. 7 3% 6 8. 63% , 0. 59% 4. 60% 7. 43% 3 , 30 d , , 21220 h 60. 56% 61. 94% 73. 07% ( 2 h) 1220 h , , [ ] ; ; [ ] R 383. 15 [ ] B Evaluation of artificial digestion m ethod on inspection ofm eat for Tr ich inel la sp ira lis contam ination and influence of the m ethod on m uscle larvae re covery Wang Guoy ing, D u J ingf ang, D un Guoqing, Sun Weili, Wang Jinx i Immunology Institute of M ed ical Colleg e, H enan Un iversity, K aif eng 475004, C ina [ Abstract] Objective T o eva luate the e e ct o arti ic ial d igestion m ethod on inspection o m eat or Tric inella sp iralis con tam ination and its in luence on activ ity and in ectiv ity o m u sc le larv ae. M ethods T he m ice w ere inoculated o ra lly w ith 100 mu scle larvae o T. sp iralis and sacr i iced on the 30 th day ollow ing the in ection. T he m u sc le larv ae o T. sp iralis w ere recov ered by three di erent test pro toco ls em p loy ing va riations o the a rti ic ia l dig estion m e thod, i. e. the irst te st proto co l eva luating d i gestion o r 2 hours ( m agne tic stirre r m ethod) , the second test pro toco l eva lua ting d igestion o r 12 hours, and the th ird te st pro to co l eva luating d igestion o r 20 hours. Each test g roup inc luded ten sam ples, and each o w h ich included 300 encapsu lated lar vae. M eanw hile, the activ ity o the recovered mu sc