dd梅花鹿生茸骨膜干细胞可溶性总蛋白抗体的制备及.pdfVIP

特 产 研 究 Special Wild Economic Animal and Plant Research 1 文章编号：1001- 4721（2010）01- 0001- 03 梅花鹿生茸骨膜干细胞可溶性总蛋白抗体的制备及滴度测定 赵海平，杨福合，邢秀梅，崔学哲，李春义※ （中国农业科学院特产研究所，吉林 吉林 132109） 摘要：为了获取梅花鹿生茸骨膜干细胞可溶性总蛋白抗体。本试验培养了梅花鹿生茸骨膜干细胞，从该细胞中提取可溶性总蛋 白。以其可溶性总蛋白为抗原，通过淋巴结和皮下注射两种方式免疫试验动物，注射的抗原量分别为70μg/ 只和280 μg/ 只。 分别于末次免疫后第 10 天和第20 天采血，分离血清，通过ELISA 试验检测血清中抗体滴度。结果表明，280 μg 皮下免疫和 70μg 淋巴结免疫均获得了较好的免疫效果，末次免疫后第10 天的抗体滴度较高。 关键词：梅花鹿；生茸骨膜；鹿茸干细胞；抗体 + 中图分类号：S865.4 2 ；S852.5 文献标识码：A Preparation and Detection of Total Soluble Protein Antibody for Antlerogenic Periosteum Stem Cells in Sika Deer （Cervus nippon Temminck） ZHAO Hai-ping ，YANG Fu-he ，XING Xiu-mei ，CUI Xue-zhe ，LI Chun-yi※ （Institute of Special wild Economic Animals and Plants ，CAAS，Jilin 132019 ，China） Abstract ：Sika deer （Cervus nippon Temminck）antlerogenic periosteal （AP）cells were cultured in order to produce polyclonal antibody. Total soluble protein of AP cells was extracted as antigen. New Zealand white rabbits were inoculated through lymph node injection or subcutaneous injection. The dosage of injection was 70μg and 280μg respectively. The blood was collected 10 days and 20 days after the last inoculation respectively ，and the serum thereof was isolated. The antibody titer of each serum was detected by ELISA. The re- sults showed that 70μg antigen lymph node injection and 280μg antigen subcutaneous injection were resulted good immune-reac- tion ，antibody titer of the serum collected 10 days after the last inoculation was higher than that of 20 days. Key words ：sika deer；antlerogenic periosteum ；stem cell；antibody 鹿茸是我国名贵的传统中药，其药用价值一直受 每天可达2cm，不会出现癌变的迹象；3）鹿茸具有很强 到人们的青睐。近年来，人们逐渐意识到鹿茸拥有更为 的免疫力，无论是脱掉鹿角后的伤口还是创伤，自然状 惊奇的生物学现象：1）鹿茸是唯一能够完全再生的哺 态下几乎不会感染。研究人员一直致力于寻找与上述 乳动物器官；2）鹿茸具有令人惊奇的