mtDNA中CO_分子标记在常见食尸性蝇类鉴定中的应用_英文_.pdfVIP

( ) 昆 虫 学 报 Acta Entomologica Sinica , June 2005 , 48 3 : 380 - 385 ISSN The availability of mitochondrial DNA cytochrome oxidase Ⅰ gene for the distinction of forensically important flies in China 1 1 1 2 3 CAI JiFeng , LIU Min , YING BinWu , DENG RengLi , DONGJianGuo , 1 1 1 1 1 ZHANGLin , TAO Tao , PAN HongFu , YAN HongTao , LIAO ZhiGang ( 1 West China School of Preclinical and Forensic Medicine , Sichuan University , Chengdu 610041 , China ; 2 West China School of Clinical Medicine , Sichuan University , Chengdu 610041 , China ; 3 School of Preclinical Medicine , Shanghai Second Medical University , Shanghai 200025 , China) Abstract : Sarcosaphagous flies are frequently found on dead bodies shortly after death. Species within this family differ in their developmental times , so an accurate identification of every species is necessary for the correct estimation of the post mortem interval ( PMI) . Identification of specimens is traditionally performed using their morphological features. The morphological similarity of sarcosaphagous flies especially their eggs , larva and pupae , poses a challenge for forensic entomologists. Therefore a molecular method was established for species identification. In this study , A 278 base pair region of mitochondri