一株热带假丝酵母木糖醇高产新菌株的筛选.pdfVIP

※生物工程 食品科学 2007, Vol. 28, No. 06 193 一株热带假丝酵母木糖醇高产新菌株的筛选 张厚瑞，赵肃清，蔡爱华，曾健智，何成新 (广西植物研究所，广西 桂林 541006) 摘 要：自木糖厂环境样品中分离出一株编号为1-18的产木糖醇分离物。在初始木糖浓度200g/L的条件下重复 循环利用1-18细胞摇瓶发酵，木糖醇转化率高达0.91(木糖醇g/木糖g)，接近理论极限值；木糖醇生成速率达 6.7g/L·h。无论产物浓度，产物生成速率与产物转化率都达到了令人满意的高水平。1-18发酵液以40ml/kg·d 的最大剂量饲喂小白鼠一周，受试动物未出现中毒反应。分离物 1-18在木糖醇生产中显然具有重要的生物工程应 用潜力。根据常规形态鉴别，生理生化实验测定，以及26S rDNA D1/D2 区域核酸序列同源性比较分析，证实 分离物1-18属于Candida tropicalis (热带假丝酵母)。分离物1-18已保存于中国典型培养物保藏中心，保藏编号CCTCC M 205067，其26S rDNA D1/D2区域核酸序列在GenBank的核酸序列登记号为DQ176428。 关键词：热带假丝酵母；木糖醇；发酵 Good Strain Screened from Candida tropicalis to Produce Xylitol ZHANG Hou-rui，Zhao Su-qing，CAI Ai-hua，ZENG Jian-zhi，HE Cheng-xin (Guangxi Institute of Botany, Guilin 541006, China) Abstracts ：The yeast isolate with a laboratory code of 1-18 cell was isolated from the environmental samples of a xylose manufacturing factory. The volumetric productivity of 6.7 g/L·h and xylitol yield of 0.91 g/g were reached respectively at initial xlylose concentration of 200 g/L by 1-18 isolate cell recycle fermentation. It reached a theoretical yield of xylitol from D-xylose. It is a satisfactory high xylitol production rate and xylitol yield. The test of acute toxicity on mice showed that the fermentation broth of isolated 1-18 cell does not show any toxic reaction at the maximum dosage of 40 ml/kg·d for one week. These results showed that the considerable biotechnological potential usage of 1-18 isolate exists for xylitol manufacture. On the bases of identification and assimilation physiological tests, its molecular characteristics was assayed by PCR conventional morphological amplification and sequencing o