白消安诱导构建胎鼠肢体畸形模型研究.pdfVIP

白消安诱导构建胎鼠肢体畸形模型的研究 哈尔滨医科大学附属第二医院整形外科(150086)宋燕妮庞建华关德宏王冰 摘要：目的建立白消安诱导SD大鼠产生后肢纵列缺损畸形仔鼠的动物模型。方法将30只孕鼠在 等多种畸形。HE染色见后肢纵列缺损较其它畸形出现率高有显著差异PO．05。骨骼X线片见骨骼变形、 缺损、腓骨胫骨化，关节扭曲等表现。结论 自消安诱导SD仔鼠四肢畸形出现多种表型，其中后肢纵列缺 损出现率最多且程度最严重。此研究为阐明纵列缺损的发生过程提供理论依据。 Inductionof ofthetibiainSDrats longitudinaldeficiency treatedwithBusulfaninutero SONG of and Jian—hua，GUAN a1．Department Yan—ni，Pang De—hong，et orthopaediac affiliatedofHarbinMedical Harbin150086China hand|2nd hospital Universityl l To ofthetibiaSDrats model Abstzact=abjeCLivestudylongitudinaldeficiency experimental isestablishedadministeredbusulfan．MethodThe30SD ratsareadministeredwith by pregnant busulfanon 12of andthe immatureratsareobserved．Tostatistics 25mg／kg day gestationabnormity whensuitabledoseisselectedto limbsand abnormalitiesof mortality a3哪姆re digit appearance， skeleton andHE—stainedwithnormalrats．BesultThe deformationslikeas X-rayplate multiple andmainfourcheare inanteriorlimb polydactylism，ankylodactylism，ectrodactylismproduced limb．The of tibiaand have and the posterior congenitallongitudinaldeficiency ectrodactyly raitointhoselimbsmalformation尸0．05．Thedifferencesare the high displayedby andHE—stainedof ratsfrcm X—ray deformity the appearance，skeleton