Gene identification and functional analysis by transgeni.pptVIP

* Gene identification and functional analysis by transgenic approach T-DNA mediated insertional mutagenesis Wild type gene RB LB T-DNA Why T-DNA? Homologous Recombination in plants is inefficient. Active transposons do not exist in every species including Arabidopsis. Agrobacterium-mediated transformation is simpler: floral dip, leaf disk transformation. More likely to generate simpler full-length integration as compared to other methods. T-DNA insertion mainly occurs in transcriptionally active area of the genome. The mutated locus gets tagged. Effect of tissue culture process on T-DNA taggin