Genome Sequencing.pptVIP

Genome Sequencing                     * Bacteriophage fX174, the first genome to be sequenced, is a viral genome with only 5,368 base pairs (bp). Fred Sanger invented shotgun sequencing, a strategy based on the isolation of random pieces of DNA from the host genome to be used as primers for the PCR amplification of the entire genome. The amplified portions of DNA are assembled in silico by their overlapping regions to form contiguous transcripts (otherwise known as contigs). The final step involved using custom primers to elucidate ga