Principles of cloning, vectors and cloning strategies.pptVIP

Principles of cloning, vectors and cloning strategies DNA CLONING DNA cloning is a technique for reproducing DNA fragments.? It can be achieved by two different approaches:? ? cell based ? ? using polymerase chain reaction (PCR).? a vector is required to carry the DNA fragment of interest into the host cell.? ?? DNA CLONING DNA cloning allows a copy of any specific part of a DNA (or RNA) sequence to be selected among many others and produced in an unlimited amount. This technique is the first stage of most of the genetic engineering experiments: ? production of DNA libraries