Promoters.pptVIP

Promoters Map ends of mRNA on DNA Mapping sites on DNA for protein binding General Properties of promoters Bacterial Promoters Promoters for eukaryotic RNA polymerases Nuclease Protection : Map the nucleotide in DNA that encodes the 5’ end of mRNA. Nuclease Protection : Map the nucleotide in DNA that encodes the 3’ end of mRNA. Primer Extension : Another method to determine DNA sequence encoding the 5’ end of mRNA Rapid amplification of 5’ cDNA ends = 5’ RACE Methods for identifying the sites in DNA to which proteins bind in a sequence-specific manner Electrophoretic Mobility Shift Assays