Recombinant DNA Technology.pptVIP

Recombinant DNA Technology rDNA Technology Restriction Enzymes and DNA Ligase Plasmid Cloning Vectors Transformation of Bacteria Blotting Techniques Restriction Enzymes Most significant advancement permitting rDNA manipulation Differ from other nucleases recognize and cleave a specific DNA sequence (Type II restriction enzymes) Restriction Enzymes Nomenclature EcoRI E = Escherichia genus name co = coli species name R = strain RY12 strain or serotype I = Roman numeral one = first enzyme HinDIII Haemophilus influenza serotype d 3rd enzyme Restriction Enzymes Recognition sites Gene