山东牛心柿群体遗传多样性与其遗传结构的研究.pdfVIP

10 山东牛心柿群体遗传多样性及其遗传结构的研究 艾呈祥，秦志华，王 洁  山东省果树研究所，山东泰安 271000  摘要：采用 SSR 分子标记技术对山东省内的 8 个牛心柿群体共 207 个个体的遗传多样性水平 及群体遗传结构进行了研究。从 51 对 SSR 引物中筛选出 10 对扩增谱带清晰和多态性丰富的 引物。10 对 SSR 引物共检测到 128 个位点，其中 105 个位点为多态位点，占 82% 。Nei’s 遗 传多样性指数（He）在0.1538~0.2476 之间，Shannon 信息指数（H0）在0.2217~0.3721 。群 体间的遗传变异占总遗传变异的 24.17%  （P  0.001 ），群体间的遗传距离（D 0.2317 ）、遗 传一致度（I 0.7832 ）、基因分化系数（φST = 0.2226 ）和遗传分化系数（FST = 0.1759 ）均表 明各群体间产生了一定程度的遗传分化。山东牛心柿群体遗传背景丰富，群体间一定程度的 遗传分化可能是人为选择压力所引起。  关键词：牛心柿；SSR ；遗传多样性；群体遗传结构  Assessment of genetic diversity and genetic structure in oriental persimmon (Diospyros kaki) populations from Shandong province using SSR markers AI Cheng-xiang, QIN Zhi-hua, WANG Jie (Shandong Institute of Pomology, Tai’ an, Shandong, 271000, China) Abstract: The genetic diversity of 207 indivdiuals from 8 persimmon (Diospyros kaki) populations in Shandong were investigated using 10 SSR markers with distinct band and abundant polymorphism selected from 51 markers. As a result, 128 bands were amplified by 10 SSR primers, of which 105 were polymorphic loci. The relatively high levels of PPL (82%), Nei’s gene diversity (He ranged from 0.1538 to 0.2476) and Shannon’s information diversity (H0 ranged from 0.2217 to 0.3721) revealed relatively rich genetic diversity in the 8 persimmon populations. Analysis of molecular variance (AMOVA) showed that the variance component of between populations contributes 24.17% at a significantly high level (P0.001). Furthermore, a low level of genetic variation among populations was detected based on the genetic distance (D 0.2317), identity (I 0.7832). Nei’s genetic diversity analysis and AMOVA revealed that there was a higher level of genetic differentiation between populations (φST = 0.2226, FST = 0.1759). The ori