应用改进的免疫荧光组织化学染色法对水稻根尖细胞微管结构的观察.pdfVIP

山东 农业 科 学 2010, 4: 40 ~ 42, 55 Sh andong A gricu ltural Sc iences 应用改进的免疫荧光组织化学染色法 对水稻根尖细胞微管结构的观察 1, 2 2 1, 3 2* 计淑霞 , 盖国琛, 戴绍军 , 刘 炜 ( 1, 150025 2 /, 250 100 3, 150040) : , , , , , : : S511 Q 245 : A : 1001- 4 94 2( 2010) 04 - 0040- 04 An Improved Immunofluorescence StainingM ethod for Observing M icrotubule Cytoskeleton in Root T ip of R ice 1, 2 2 1, 3 2* JI Shu - x ia , GA I Guo- chen , DA I Shao - jun , L IU W ei ( 1. College of Lfi e S cience and Technology, HarbinN orma l University, H arbin 150025, China; 2. H igh- Tech Research Cen ter, Shandong A cademy of Agric lt ral Sciences/ K ey Laboratory of Crop Genetic Imp rovem ent and B iotechnology, H angh aihai, M inistry of Agric lt re, The Peop lesR ep blic of China, Jinan 250 100, China; 3. College of Lfie Science, N ortheast Forestry University, H arbin 150040, China) Abs tract T he m icrotubule (MT ) cytoske leton plays an mi portant role in p lant cell m orphogenesis. A s the vo lum e o fMT is very sm a ll, and itsm orpho logy is a wl ays in dynam ic changes, it is very d ifficu lt to ob serve a clear pattern o f m icro tubule. In th is pap er, an mi proved mi munofluorescence sta in ing m ethod w as introdu ced u sing the roo t t ip of rice as expermi ental m ater ia ls, and the results show ed that a clearly and fine structure o f m icrotubu le cy toskeleton could be ob served. T his m ethod w ill play a gu iding role in m icro tubule cytoske leton invest igation and observation of other p lant ce lls. K ey w ords P lant cell M i