NaCl处理对油菜单倍体和二倍体茎端组织生长的影响.pdfVIP

2008, 24( 2): 25- 28 A cta Agriculturae Shanghai : 1000-3924( 2008) 02-25-04 NaCl 王亦菲, 单丽丽, 周润梅, 陆瑞菊* (; , 201106) : : 210521072109 2117 , N aC l : 0. 4% NaCl; 0. 8% NaC l, NaC l 1. 2% ~ 1. 4% , NaC l : ; ; ; ; NaC;l : S565. 4; 945. 78 : A The effects ofNaCl treatm ent on haploid and diploid stem apexes growth of rape * WANG Y i-fei, SHAN L-i li, ZHOU Run-m ei, LU Ru i-ju (B iotech R esearch Institute, ShanghaiA cademy of Agricultural Sciences; ShanghaiK ey Lab of Agricultural Genetics and B reeding, Shangha i 201106, China) Abstract: T em icrospore-derived aploid plantlets and seed-germ inated diploid plantlets ofBrassica na us w ere used as testm aterials, and t e in vitro cu lture of t eir stem apexesw as conducted underNaCl stress. T e resu lt s ow ed t at adding 0. 4% N aC l to med ium could lead t e increment rate of donorm aterials fres w eig t to rise, add ing 0. 8% NaCl to med ium could significantly decrease t emu ltiplication rate of donorm aterials, and w en t e N aCl concentration increased to 1. 2% ~ 1. 4% , t e survival rate of donor materials obviously de- creased. T e in ib ition ofN aCl stress on growt of aploid materialsw as greater t an t at on grow t of d ip loid ones. Key words: Brassica na us; H ap loid; D ip loid; Stem apex; N aCl; Stresss [ 1, 2] , , [ 3, 4] , , [ 5- 7] () (), , [ 8] , , NaCl , 1 1. 1 2105, 2107, 2109, 2117 : 2007- 11- 20 ; 2008- 05- 15 : ( 05ZR 14104) : ( 1968- ),