NF_B的诱捕寡核苷酸抑制肺纤维细胞_型胶原mRNA表达的实验研究.pdfVIP

∀ 322 ∀ 24 3 2008 5 IMMUNOLOGICAL JOURNAL Vol. 24 No. 3 May. 2008 [ ]2008 NFBmRNA * 杨雪梅, 王兴胜 , 崔社怀 (第三军医大学大坪医院野战外科研究所呼吸内科,重庆400042) [ ] NFB (decoyoligonucleotides,DecoyODNs) , NFB DecoyODNs, ,( electrophoretic mobility shift assay, EMSA) DecoyODNs NFB ,RTPCR NFBDecoyODNs NFB, DecoyODNs 18 h , mRNA DecoyODNsNFB , [] ;NFB; ; [] R135.2 [ ] A Inhibitingeffects ofNFB decoy oligonucleotides on the mRNA expression offibroblast type I col lagen in primary murine lung fibroblasts YANG Xuemei, WANG Xingsheng, CUIShehuai (StateKey Laboratory of Trauma, Burns and Combined Inj ury , De p artment of Respiratory, Institute of Surgery Research, Daping Hospital, Third Military Medical niversity, Chongqing 400042, China) [Abstract] Objective To investigate the effects of NFB decoyoligonucleotides (decoyODNs) on the expression of fibroblast type I collagen forprovidingtheatheoreticalbasisfor pulmonary fibrosis prevention. Methods DecoyODNs targetingNFBwere designed andsyn thesized. Primary murine lung fibroblasts weretransfected with cationic liposomes. The inhibiting effects of decoyODNs on NFBwere deter mined by electrophoreticmobility shift assay (EMSA) . And the effects of decoyODNs on the type I collagen synthesis in the lung fibroblasts were analyzed by RTPCR. Results NFB decoyODNs could competitively inhibit the activity of NFB in vitro. ThemRNA expression of fibroblast type I collagen decreased evidently after 18 h of DecoyODNs treatment. Conclusion NFB decoyODNs could inhibit themRNA expression of fibroblast type I collagen, which might provide atheoretical basis for pulmonaryfibrosis prevention. [ ey words] Decoyoligonucleotides; NFB; Fibroblast type I collagen; Fibroblasts ( idiopathic pul