饮用水中几种细菌计数方法的比较.pdfVIP

第 25 卷第 4 期 环 　　境 　　科 　　学 Vol . 25 ,No . 4 2004 年 7 月 EN V IRON M EN TAL SCIEN CE J uly ,2004 饮用水中几种细菌计数方法的比较 鲁巍 ,王云 ,张晓健 (清华大学环境科学与工程系 ,北京 　100084) ( ) 摘要 : 比较采用不同培养基的平板计数 Plate Count s ,PC 方法 , 以及不同荧光染色剂的显微镜直接计数方法与常规计数方法 ( 的差别. 研究认为 ,常规平板计数方法并不能准确反映饮用水中实际存活的细菌数量 ;吖啶橙直接计数 Acridine Oran ge Direct ) ( ) Count s ,AODC 的结果最高 ,较常规平板计数方法高出 3～4 个量级 ;活细菌直接计数 Direct Viable Count s ,DVC 中 ,DVCN . A . 、DVCCTC 和 DVCBacLight 等计数方法的结果较常规平板计数结果高出 2 ～3 个量级. 以地表水为水源的水厂出水中活 细菌数占总细菌数的比例在 10 %左右. 关键词 :饮用水 ;直接计数 ;平板计数 ;活细菌直接计数 ;细菌 ( ) 中图分类号 :X832 ; TU99 125 　文献标识码 :A 　文章编号 :0250330 1 2004 040 16703 Methods of Enumeration of Bacteria in Drinking Water L U Wei ,WAN G Yun , ZHAN G Xiaoj ian (Dep art ment of Environmental Science and Engineering , Tsinghua University , Beijing 100084 , China) Abstract :Met hods of t he enumeration of total bacteria and Coliform in drinking water were researched in t his p ap er . The differences between heterotrop hic plate count and direct viable count met hod were comp ared . It is concluded t hat t he total number of bacteria in R2A medium is one order of quantity higher t han t he traditional plate count agar , and t he result s of acridine orange direct count s (AODC) is t he highest . Some different staining met hods in direct viable count were also comp ared in t his p ap er , such as nalidixic acid , CTC staining and BacLight staining. The proportion of t he live bacteria to t he dead is about 10 %. Key