分离应用研究.pdfVIP

Journal of Chromatography A, 1235 (2012) 34–38 Contents lists available at SciVerse ScienceDirect Journal of Chromatography A j o u r n a l h o me p a g e : w w w. elsev /locate/chro ma Application of step-wise gradient high-performance counter-current chromatography for rapid preparative separation and purification of diterpene components from Pseudolarix kaempferi Gordon Shichao He a,b , Shucai Li a , Jianhong Yang a , Haoyu Ye a , Shijie Zhong a , Hang Song b , Yongkui Zhang b , Cheng Peng c , Aihua Peng a,c,∗ , Lijuan Chen a,b,∗ a State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China b College of Chemical Engineering, Sichuan University, Chengdu 610065, China c State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China a r t i c l e i n f o a b s t r a c t Article history: In general, simultaneously separation and purification of components with a broad polarity range from Received 19 October 2011 traditional Chinese medicine (TCM) is a challenge by an ordinary high-speed counter-current chro- Received in revised form 7 January 2012 matography (HSCCC) method. In this paper, we describes a rapid and efficient separation method of Accepted 15 January 2012 combining three-step gradient elution and two-step flow-rate gradient elution using high-performance Available online 9 February 2012 c