激素对百合植株再生的影响.pdfVIP

Guihaia 22 ( 2) : 167 - 170 2002 3 刘明志, 林雪艳 ( , 510632 ) : 15 2,4- 6- D BA : MS ,BA , 1. 02.0mg/ L BA , 6. 0 mg/ L BA ; 2, 4-D , 4. 0 mg/ L 2, 4- , 6. 0 / 2, 4- ; D mg L D BA 2, 4-D , , MS 1.0 / 2.0 / , , mg L mg L IAA M S MS : ; ; : Q 42. 5: A : 1000-3142(2002) 02-0167-04 Ef fects of hormone on plant regeneration of lily (Lilium daviaii Duch) LIU M ing-zhi LIN Xue-yan ( Dep ar tm ent of B iotech nology , J inan Univ er sity , Guangzhou 510632, China ) Abstract: Somatic embryogenesis and organogenesis of scale leaf of lily on medium containing 15 kinds of dif - 2, 4- . ferent combination of BA and D were obtained to study the eff ect of hormone on plant regeneration Re- sults show that BA in M S medium can induce direct adventitious bud f ormation, and when the concentration 1.0 / 2. 0 / , of BA is between mg L and mg L the highest frequencies of adventitious bud diff erentiation can be acquired. 2, 4-D owns the ability to induce direct somatic embryogenesis and 4. 0 mg/ L 2, 4-D can induced the highest frequency of direct somatic embryogenesis, but 6.0 mg/ L 2,4-D maybe cause somewhat inhibito- ry effect s on direct somatic embryogenesis. Both of adventitious bud and direct somatic embryogenesis can be attained when BA and 2, 4-D are utilized in culture medium simultaneously. Only root f ormation can be in- - - duced on