富马酸酮替芬毛细管电泳电化学发光法的研究.pdfVIP

′06 陕西药物分析学术研讨会 富马酸酮替芬毛细管电泳电化学发光法的研究 孙四娟，唐玉海，熊凤梅，王楠楠，熊迅宇 （西安交通大学理学院分析科学研究所，西安，710061 ） 摘要 采用毛细管电泳——钌联吡啶电化学发光法检测了制剂中富马酸酮替芬的含量。讨论 了钌联吡啶浓度、检测电位、进样高压及时间、检测池中及运行磷酸缓冲液的浓度及pH 值、 分离高压等条件对富马酸酮替芬电化学发光强度的影响。在优化的实验条件下，富马酸酮替 -5 -7 -1 芬在 1.0×10 ～1.0×10 g ·ml 范围内呈良好线性关系（相关系数分别为 0.993 ）；检出限 -8 -1 -6 -1 别为 3 ×10 g ·ml ，对 1.0×10 g ·ml 的富马酸酮替芬进行 11 次平行测定，其峰高和迁 移时间的 RSD 分别为 5.22%，0.98%.该方法已成功用于富马酸酮替芬片及胶囊含量的测定， 其回收率为分别为 105.0%，101.3%。 关键词 毛细管电泳；电化学发光；富马酸酮替芬；制剂 Determination of Ketotifen Fumarate by Capillary Electrophoresis with Electrochemiluminescence SUN Si-juan, TANG Yu-hai, XIONG Feng-mei, WANG Nan-nan, XIONG Xun-yu (Institute of Analytical Sciences, Xi’an Jiaotong University, Xi’an, 710061,China) 2+ Abstract Capillary electrophoresis coupled with electrochemiluminescence based on Ru(bry)3 was developed for the determination of ketotifen fumarate.. Conditions affecting the detection and 2+ separation, such as the concentration of Ru(bpy)3 , detection voltage, injection time and voltage, the concentration and pH of phosphate buffer in detection cell and running buffer, separation voltage was investigated. Under the optimized conditions, the linear range of ketotifen fumarate -5 -8 -1 -8 -1 was 1.0×10 ～1.0×10 g·ml ( r=0.993), the limit of detection was 3×10 g·ml , the RSD for ECL intensity and migration time were 5.22% ，0.98%, respectively, obtained from eleven times consecutive injection. The method was successfully applied for the determination of ketotifen