N-乙酰半胱氨酸对砷致小鼠脂质过氧化损伤的保护作用研究.pdfVIP

武汉华中农业大学 2006．10 N．乙酰半胱氨酸对砷致小鼠脂质过氧化损伤的保护作用研究 向金梅¨，郭定宗‘，杨世锦‘ (1华中农业大学动物医学院湖北武汉430070；2湖北生物科技职业学院湖北武汉430070) f摘要】目的探讨N-乙酰半胱氨酸(NAc)对砷致小鼠脂质过氧化损伤的拮抗作用．方法40只昆明小 鼠随机分为5组：对照组，单纯染砷组，低、中、高剂量NAC处理组．染毒8周后处死小鼠，取其血液和 戊酸脱水酶(6-ALAD)活性和MDA、Hb含量．结果与对照组相比，单纯染砷组小鼠抗氧化酶活性显著 的产生和脂质过氧化反应，提高机体抗氧化酶活性，从而发挥其对机体的保护作用． 【关犍词】砷；N一乙酰半胱氨酸；抗氧化酶；脂质过氧化 Onthe effectof andⅥtEon induced Study protectiveN-acetyl-cysteinelipidperoxidationdamage by arsenicinrat JinmeiGuo Xiang Dingzon91YangShijm (1 ofanimal College veterinary,Huazhongagricultural (2 ofHubei College biotechnology,Wuhan，Hubei。China) AbstractITheaimofthe was actionof onthe to the experimentstudyantagonisticN．acetyl-cysteinelipid inducedarsenicin and rats．40 ratsweredividedintofive of peroxidationdamage by blood,liverkidney Kunming oflow doseand weeks dose，medial dose．Eightlater, groups：control，arsenicexposedgroup，NACgroups high allratswerekilledtoexaminethe antioxidase and ofsomebiochemical SOD。GSH．Px，CAT changes indices(the the of MDAinliverand and in SOD，6-ALAD，Hb，MDA productionlipidperoxidation kidney blood)．The resultsshowedas withthoseof antioxidasesofarsenic control，the activity group fellows：(1)Compared