学士学位论文：茶渣提取液对淡水藻类生长影响的研究.docVIP

学士学位论文：茶渣提取液对淡水藻类生长影响的研究 学士学位论文：茶渣提取液对淡水藻类生长影响的研究 学士学位论文：茶渣提取液对淡水藻类生长影响的研究:2013-5-14 14:57:57学士学位论文题目： 茶渣提取液对淡水藻类生长影响的研究 院（系） 化工学院 专 业 环境工程 届 别 2012届 茶渣提取液对淡水藻类生长影响的研究摘要本论文以蛋白核小球藻，斜生栅藻和水华微囊藻为实验材料，通过分析茶渣提取液对蛋白核小球藻，斜生栅藻和水华微囊藻藻密度、叶绿素含量、过氧化氢酶(CAT)以及超氧化岐化酶(SOD)活性和脂质过氧化丙二醛(MDA)含量的变化，研究了其中的化感物质对蛋白核小球藻、斜生栅藻和水华微囊藻生长的影响，并讨了茶渣提取液对三种淡水藻类的化感作用机理。研究发现茶渣提取液中的化感物质对蛋白核小球藻、斜生栅藻和水华微囊藻有抑制作用，且随着其浓度的增大，三种藻受到的抑制作用越明显，这表明茶渣提取液对藻类的化感作用具有一定的浓度依赖性。蛋白核小球藻、斜生栅藻和水华微囊藻96h半效应浓度EC50值分别7.48 g/L、0.826 g/L和0.533 g/L。茶渣中的化感物质使三种藻的藻密度、叶绿素含量降低，使CAT、SOD酶活和MDA含量升高。由以上结果可以推测氧化损伤可能是茶渣中的化感物质对蛋白核小球藻、斜生栅藻和水华微囊藻生长的化感作用重要机理之一。关键词：茶渣提取液；化感物质；淡水藻类；氧化损伤 Tea-leaf extracts of freshwater algae growth impact studyAbstract In this thesis, Chlorella pyrenoidosa, Scenedesmus obliquus and Microcystis flos-aquae as experimental materials to explore the allelopathic matter in the tea-leaf extract of Chlorella pyrenoidosa, Scenedesmus obliquus and the inhibition of Microcystis flos-aquae, on this basis, further study of allelochemicals on the chlorophyll content of Chlorella pyrenoidosa, Scenedesmus obliquus and Microcystis flos-aquae, catalase (CAT) and superoxide Qidismutase (SOD) activity and lipid oxidation of malondialdehyde (MDA), and speculated that the mechanism of the tea-leaf extracts on allelopathic effects of three kinds of algae. Allelochemicals in the tea-leaf extract of Chlorella pyrenoidosa, Scenedesmus obliquus and Microcystis flos-aquae sp inhibition, and with the increase of its concentration, the inhibitory effect of three kinds of algae are more obvious, 96h half-effect concentration values of EC50 were7.48 g/L,0.826 g/L and 0.533 g/L. Tea dregs of allelochemicals to make three kinds of algae chlorophyll content decreased, while the CAT, SOD and MDA were increased. From the above results can be hypothesized that oxidative damage may be tea dregs of allelochemicals on Chlorella pyrenoidosa, Scenedesmus obliquus and Microcystis flos-aqu