胃癌及其癌前病变中ras，c┐erbB┐2，p53癌基因产物的表达_医学论文.docVIP

胃癌及其癌前病变中ras，c┐erbB┐2，p53癌基因产物的表达_医学论文 胃癌及其癌前病变中ras，c┐erbB┐2，p53癌基因产物的表达_医学论文 作者：惠延平　黄高升　王文亮　王映梅　朱晓慧　马福成 【关键词】 胃肿瘤 　　关键词: 胃肿瘤癌前状态基因表达免疫组织化学 　　摘 要:目的 探讨胃癌及其癌前病变中ras，c-erbB-2，p53癌基因蛋白产物的表达分布特点及其在胃癌发生发展中的作用. 方法 应用免疫组化ABC方法，对58例胃癌、31例胃粘膜上皮异型增生和11例正常胃粘膜组织中，鼠抗人mAb ras基因p21蛋白、兔抗人c-erbB-2原癌基因产物多克隆抗体p185，鼠抗人mAb p53蛋白进行了检测. 结果 p21在正常胃粘膜和轻、中、重度异型增生和胃癌中的表达率分别为27.3%，30.0%，46.2%，75.0%和72.4%其表达率在重度异型增生和胃癌中显著高于正常胃粘膜和轻、中度异型增生（0.05）p185在正常胃粘膜和轻、中度异型增生中无表达，在重度异型增生和癌中的阳性表达率分别为25.0%，18.9%，各组间无显著性差异（Pgt0.05）p53仅在癌中有表达，表达率29.3%，明显高于非癌组织（0.05）.胃癌中两种以上癌基因蛋白同时表达率明显高于非癌组织（0.05）. 结论 ras基因可能作用于胃癌发生的早期阶段，c-erbB-2，p53基因可能作用于胃癌发生的较晚期.并认为胃癌发生需要多基因协同作用. 　　Keywords:stomach neoplasmprocancerous conditiongene expressionimmunohistochemistry Abstract:AIM To investigate the expression and distribu-tion features of p21，p53and p185oncoproteins in gastric cancer and its precancerous lesions.METHODS Immuno-histochemical method was used to measure tissues of58gas-tric cancer and31gastric mucosa dysplasia.RESULTS Pos-itive rate of p21in mild，moderate，severe dysplasia and can-cer of stomach was3/10（30.0%），6/13（46.2%），6/8（75.0%）and42/58（72.4%）respectivelythe positive rate of p185in severe dysplasia and cancer of stomach was2/8（25.0%）and11/58（18.9%）resprectivelyp53expression was only in cancer17/58（29.3%）.The positive percentage of ras p21in severe dysplasia was significantly higher than that of normal mucosa or mild dysplasia（0.05），ap-proaching the level in carcinoma（Pgt0.05）.The positive ex-pression percentage of p53in gastric carcinoma was signifi-cantly higher than that of non-carcinomarous tissue（0.05）.There were more than two kinds of oncogenes overex-pressed in gastric carcinoma（0.05）.CONCLUSION Over-expression of p21，p185，p53may play an important role in the onset and development of gastric carcinomathe ras gene product may be correlated to the events happenning in the early period of gastric carcinogenesis，while p185and p53gene proteins may be related to the later period of gastric carcinogenesi