脂联素基因启动子区_医学论文.docVIP

脂联素基因启动子区_医学论文 脂联素基因启动子区_医学论文 作者：何岚，刘萍，叶枫，李建宁，董春萍 【关键词】 脂联素；单核苷酸多态性；2型糖尿病；胰岛素抵抗 　　Association of SNPs in proximal promoter region of APM1 gene with serum adiponectin level and type 2 diabetes in Shaanxi Chinese 　　【Abstract】 AIM: To study the correlation between single nucleotide polymorphisms (SNPs)11377C/G and serum adiponectin level in the Han population with type 2 diabetes in Shaanxi province. METHODS: The PCR RFLP techniques were adopted to test SNPs in the proximal promoter region of the APM1 gene in 101 nondiabetes control group and 203 diabetes group. The diabetes patients underwent 75 g glucose OGTT and insulin release test for measurement of serum adiponectin, blood sugar, insulin and lipids. Insulin secretion index and insulin resistance index were calculated. RESULTS: Serum adiponectin level in the diabetes group were statistically lower than nondiabetes control group ［(12.8±6.5) mg/L vs (15.0±6.9) mg/L, 0.05］. The distribution of APM1 gene polymorphism in nondiabetes group and diabetes group was C/C53(52.5%), C/G38(37.6%), G/G10(9.9%) and C/C107(54.0%), C/G74(37.4%), G/G17(8.6%). In nondiabetes control group, serum adiponectin level of the GG genotype at position 11377 was lower than that of the CC genotype ,but the TG level of the GG genotype was much higher than that of the CC genotype (0.05). CONCLUSION: SNPs11377C/G modulate the adipocytesecreted adiponectin hormone level and may contribute to the genetic risk for type 2 diabetes in Shaanxi Chinese. 　　【Keywords】 adiponectin single nucleotide polymorphism type 2 diabetes mellitus insulin resistance 　　【摘要】目的： 探讨中国陕西地区汉族人群脂联素基因启动子区11377C/G单核苷酸多态性(SNPs11377C/G )和血清脂联素水平与2型糖尿病的关系. 方法： 研究对象304例，其中2型糖尿病203例，非糖尿病对照101例，用PCRRFLP方法鉴定SNPs11377C/G，进行了OGTT（75 g葡萄糖）及胰岛素释放实验，测定脂联素、血脂、血糖、胰岛素水平并计算Homa胰岛素分泌和抵抗指数. 结果： 2型糖尿病组血清脂联素水平明显低于非糖尿病对照组（mg/L, 12.8±6.5 vs 15.0±6.9, 0.05）. SNPs11377C/G基因型和等位基因频率在糖尿病和非糖尿病对照组间分布无统计学差异(Pgt0.05)，非糖尿病组11377 GG基因型脂联素水平明显低于CC基因型，而TG明显高于CC基因型(0.05). 结论： 脂联素基因启动子区SNPs11377C/G与陕西地区汉族人血清脂联素水