膀胱尿路上皮癌Fas相关死亡结构域蛋白表达及意义_医学论文.docVIP

膀胱尿路上皮癌Fas相关死亡结构域蛋白表达及意义_医学论文 膀胱尿路上皮癌Fas相关死亡结构域蛋白表达及意义_医学论文 【摘要】 目的 研究Fas相关死亡结构域 (FADD) 蛋白在膀胱尿路上皮癌（BUC）组织的表达，并探讨其与肿瘤生物学行为之间的关系。方法 采用免疫组化SP法检测68例BUC及13例正常膀胱黏膜组织FADD蛋白表达。原位杂交法检测其中34例BUC组织FADD mRNA 表达水平。结果 正常膀胱黏膜FADD蛋白呈阳性表达。FADD的阳性率在不同组织学分级、不同临床分期的BUC组织中差异均有极显著性(χ2=15.38、11.34，0.01)；复发组与未复发组FADD的阳性率分别为36.84%和53.33%,差异无显著意义(χ2=1.85，Pgt0.05)。BUC组织中FADD mRNA阳性率为44.12%, FADD蛋白阳性率为47.06%,两者差异无统计学意义(χ2=0.06，Pgt0.05)。结论 FADD表达异常在BUC的发展中可能起着重要作用, FADD阳性表达与BUC病理分级、浸润关系密切，但与BUC复发无关。 【关键词】 Fas相关死亡结构域蛋白；膀胱肿瘤；免疫组织化学；原位杂交 ［ABSTRACT］ Objective To investigate the Fas-associated death domain (FADD) protein and its biological behavior in bladder urothelial carcinoma (BUC). Methods The expressions of FADD in 68 BUC and 13 normal bladder tissue were detected by streptavidin-peroxidase immunohistochemical technique. Of which, 34 BUC were studied by in-situ hybridization for FADD mRNA. Results The various exprssions of FADD were as follows: it positively expressed in normal bladder mucosa the differences of positive expression were significant among different histological grading and clinical staging (χ2=15.38,11.340.01) its expression in recurrent group and non-recurrent group was 36.84% and 53.33%, respectively, the difference was insignificant (χ2=1.85,Pgt0.05). In BUC, the positive FADD mRNA was 44.12% and FADD protein was 47.06%, with no statistical difference was noted between them (χ2=0.06,Pgt0.05)。 Conclusion The abnormal expression of FADD may play an important role in the development of BUC. The positive expression of FADD in the cancer is closely related to the pathological grading and invasion, but not related to its recurrence. ［KEY WORDS］ Fas-associated death domain protein Bladder neoplasmas Immunohistochemistry In situ hybridization Fas相关死亡结构域(FADD)蛋白能与Fas相互作用而诱导细胞凋亡,在细胞凋亡信号传导通路中起重要作用,但其在膀胱尿路上皮癌（BUC）发病过程中的作用尚不清楚。我们分别采用免疫组化SP法、原位杂交法检测BUC组织中FADD蛋白及mRNA表达, 并探讨FADD与BUC生物学行为之间的关系。现报告如下。 1 材料与方法 1.1 标本 随机选取我院1999~2002年经手术切除的初发性BUC石蜡标本68例，男49例，女19例；年龄30~85岁，平均60.2岁。临床病