茶黄素对大鼠脑缺血再灌注损伤后细胞凋亡及凋亡相关基因的影响_药学论文.docVIP

茶黄素对大鼠脑缺血再灌注损伤后细胞凋亡及凋亡相关基因的影响_药学论文 茶黄素对大鼠脑缺血再灌注损伤后细胞凋亡及凋亡相关基因的影响_药学论文 作者：蔡飞 ，李彩蓉，吴基良，闵清，欧阳昌汉，余薇 【摘要】 　　目的探讨茶黄素对大鼠局灶性脑缺血再灌注损伤后细胞凋亡及凋亡相关基因的影响。方法采用线拴法建立大鼠大脑中动脉栓塞(MCAO)模型。大鼠随机分为缺血组，假手术组，茶黄素小剂量组(10 mg/kg)，茶黄素大剂量组(20 mg/kg)。缺血2h，再灌注24 h后处死动物，于再灌注前舌下静脉给药。测定大鼠神经系统症状，脑梗塞体积，脑组织MDA，NO含量以及SOD和GSH-Px活性，采用TUNEL法检测脑组织细胞凋亡，采用免疫组织化学法检测凋亡相关基因Caspase-3的表达。结果茶黄素治疗组与模型组相比神经系统症状减轻，脑梗塞体积缩小，脑组织MDA，NO含量减少，SOD和GSH-Px活性增加。脑组织细胞凋亡及Caspase-3表达减少。结论茶黄素对大鼠局灶性脑缺血再灌注损伤具有保护作用，其作用机制可能与抗氧化和抑制细胞凋亡有关。 【关键词】 茶黄素 缺血再灌注 细胞凋亡 Caspase-3 　　Abstract：ObjectiveTo study the effect of theaflavin on cell apoptosis and apoptosis related gene after focal cerebral ischemia injury in a rat model of middle cerebral artery occlusion (MCAO). MethodsMale Sprague-Dawley rats were anesthetized and subjected to 2 h of MCAO followed by 24 h reperfusion. The neurological deficits and the sizes of cerebral infarction were measured. The contents of malondialdehyde (MDA), and nitric oxide (NO) in brains were tested. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were also tested. TUNEL staining to label the neuronal apoptosis and immunohistochemistry to detect the expression of caspase-3 protein were used.ResultsThe neurological deficits and the sizes of cerebral infarction were decreased in theaflavin-treated groups. The contents of MDA and NO in theaflavin-treated groups were lower than those of the ischemic group. The activities of SOD and GSH-Px in theaflavin-treated groups were higher than that of the ischemic group. Theaflavin-treatment also decreased neuronal apoptosis and the expression of caspase-3 protein. ConclusionTheaflavin has protective effects on focal cerebral ischemic reperfusion injury through inhibiting oxidative stress and lessening apoptosis. 　　Key words：Theaflavin; Ischemia-reperfusion; Apoptosis ; Caspase-3 　　据国内外流行病学调查表明，脑血管病是一类严重威胁人类健康和寿命的常见病，多发病，是目前世界上导致人类死亡的三大主要疾病之一。在我国，脑血管病已经上升为第一位致死致残疾病。脑缺血后能量代谢障碍、细胞内Ca2+超载及其所引发的谷氨酸的兴奋性毒性反应是神经元损伤的始动环节，由此产生一系列生化反应。在脑缺血损伤中，细胞凋亡是由各种凋亡