血小板衍生生长因子受体在膀胱癌中的表达意义_医学论文.docVIP

血小板衍生生长因子受体在膀胱癌中的表达意义_医学论文 血小板衍生生长因子受体在膀胱癌中的表达意义_医学论文 【关键词】 膀胱肿瘤 　　关键词 :膀胱肿瘤受体，血小板源生长因子新生血管化，病理性免疫组织化学 　　摘 要:目的 探讨血小板衍生生长因子受体（PDGFR）表达与膀胱移行上皮细胞癌发生、生物学行为及预后的关系. 方法 采用免疫组织化学技术――两步法检测68例膀胱移行细胞癌和10例正常膀胱组织PDGFR表达及膀胱癌组织中微血管密度（MVD）. 结果 PDGFR阳性染色分布于癌细胞、间质、血管及炎症细胞的胞质及胞膜.膀胱癌组织PDGFR阳性率为83.8%，明显高于正常膀胱组织（30.0%，0.01），膀胱癌组织中PDGFR阳性表达组的微血管密度（MVD）明显高于PDGFR阴性表达组（85±30vs36±15，0.01）. 结论 PDGFR表达与正常膀胱组织生长有关，过度表达与癌的形成、血管的形成及炎症细胞功能相关，未发现PDGFR表达与肿瘤分期、分级及预后相关. 　　Keywords:bladder neoplasmsreceptors，platelet-dervied growth factorneovascularization，pathologicim-munohistochemistry 　　Abstract:AIM To study the relationship between expres-sion of platelet-derived growth factor receptor（PDGFR）and the genesis，biological behavior and prognosis of bladder transitional cell carcinoma.METHODS Expression of PDGFR and microvessel density（MVD）were investigated in68cases of transitional cell carcinoma in bladder and10cases of normal bladder tissues with immunohistochemical technique according to the procedures of classical two-steps.RESULTS It was revealed that the PDGFR positive staining rate in tumor tissues（83.8%，57/68）was significantly higher（0.01）than that in normal tissues（30%，3/10）the pos-itive staining was confined to the cytoplasms and membranes of epithelial，interstitial，vascular smooth muscle and inflam-matory cells.MVD was higher in the positive expression of PDGFR than in the negative patients（85±30vs36±15，0.01）.CONCLUSION Expression of PDGFR is related to the growth of the normal bladder tissues，but the increased expression of PDGFR is associated with malignant transfor-mation and development of bladder carcinoma，angiogenesis in the bladder cancer and the function of inflammatory cells.There might be no significant correlation between expression of PDGFR and tumor grade，stage and prognosis. 　　0 引言 　　血小板衍生生长因子（PDGF）是细胞生存微环境中的重要一员［1-3］ .研究表明，其受体（PDGFR）表达失调可导致细胞增殖及恶变［4-7］ .PDGFR在膀胱肿瘤表达的研究较少且关系尚未明确.我们采用免疫组织化学技术――两步法，在检测膀胱移行上皮细胞癌组织中PDGFR表达的同时用VWF一抗检测肿瘤的微血管密度（MVD），探讨PDGFR表达与膀胱移行细胞癌发生