野葛根和银杏叶提取物配伍复方制剂的抗氧化实验研究_药学论文.docVIP

野葛根和银杏叶提取物配伍复方制剂的抗氧化实验研究_药学论文 野葛根和银杏叶提取物配伍复方制剂的抗氧化实验研究_药学论文 作者：潘洪平，危华玲，杨嘉珍 【摘要】 目的观察银杏叶复方制剂对老龄小鼠的抗氧化作用。方法老龄小鼠40只，随机分成老龄对照组，银杏叶单方制剂组，银杏叶复方制剂高、低剂量组，每组10只。另10只标准体重小鼠作青年对照组。老龄对照组和青年对照组均给予等体积蒸馏水，各用药组连续灌胃给药10 d。末次给药后1 h，采血分离血清，检测超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量。结果与老龄对照组比较，银杏叶复方制剂高、低剂量组和单方制剂组均可明显提高老龄小鼠血清中SOD活性，降低MDA含量。银杏叶复方制剂高剂量组提高SOD活性的强度大于低剂量组和单方制剂组（0.05）。结论银杏叶复方制剂具有抗氧化作用且呈现一定的量效关系，处方中的银杏叶提取物与野葛根提取物成分具有一定的协同药效作用。 【关键词】 银杏叶复方制剂； 银杏叶提取物； 野葛根提取物； 抗氧化 China)Abstract：ObjectiveTo study the effects of compound Ginaton on SOD activity and MDA content in blood serum of aging mice.Methods40 aging mice were randomly divided into 4 groups: aging group, positive control group (tablet Ginaton), high and low dose of compound Ginaton groups, 10 mice each group. Other 10 normal mice, weight of 18～22g, were taken as youth control group. Both aging group and youth control group were administered equal distilled water. After administration for 10 days, serum of each mice in all groups was separated from the whole blood of eyeball arteries, and its SOD activity and MDA contents were detected respectively. ResultsSOD activity in blood serum was significantly higher in positive control group, high and low dose of compound Ginaton groups than that in aging control group mice. On the contrary, MDA content in blood serum of aging group was the highest among all groups. SOD activity in high dose of compound Ginaton group was significantly higher than those in low dose of compound Ginaton group and positive control group (0.05).ConclusionCompound Ginaton has the action of antioxidation and this action has an obvious dose-effect relationship. It also shows the synergism between the two main compositions of compound Ginaton. 　　Key words：Compound Ginaton； Ginaton； Pueraria extract； Antioxidation 最近研究报道，银杏叶提取物能有效清除细胞内氧自由基，对胰岛细胞凋亡有保护作用［1］；银杏叶提取物可以通过增强运动机体抗氧化酶的活性，提高机体的抗氧化功能，减轻大强度耐力训练对大鼠组织造成的脂质过氧化损伤，改善运动造成的自由基代谢紊乱，加快自由基的清除［2］。葛根提取物具有抗氧化应激的作用，可提高糖尿病大鼠血浆超氧化物歧化酶的活性，减少丙二醛的产生，有效防治糖尿病血管病变的发生［3］；葛根提取物的抗氧化作用主要与其主要有效成分