针刺加当归芍药散治疗阿尔茨海默症的机制研究-_医学论文.docVIP

针刺加当归芍药散治疗阿尔茨海默症的机制研究-_医学论文 针刺加当归芍药散治疗阿尔茨海默症的机制研究*_医学论文 作者：李忠仁，沈梅红，穆艳云，欧阳颀，马骋 【摘要】 目的 探索临床针刺加当归芍药散(DGSYS)治疗阿尔采默氏症的可能机理。方法 通过腹腔注射药物东莨菪碱或亚硝酸钠，或者用奎宁酸损毁大脑海马CA1区诱发大鼠发生记忆力障碍，造成不同类型的大鼠“痴呆”模型，对照性观察针刺、针刺加DGSYS、DGSYS及NM对记忆障碍功能的影响和与记忆相关实验指标参数的变化。结果 针刺加DGSYS疗法抗痴呆作用机制可能与调节大脑兴奋性氨基酸Glu能和胆碱能神经递质的功能，改善体内抗氧化系统的能力，如改善超氧化物歧化酶/脂质过氧化物含量及一氧化氮/一氧化氮合酶的活性比值有关，它们的协调作用促使了大脑及海马CA1区受损的痴呆模型的LTP增强、大鼠学习记忆能力的复苏，从而改善了痴呆症状。结论 针刺加DGSYS疗法治疗AD痴呆及改善学习记忆能力的作用具有确切的科学依据，值得临床推广应用。 【关键词】 针灸 当归芍药散 阿尔茨海默症 痴呆造模 神经递质 抗氧化作用 长时程增强 The studies on mechanism of Alzheimer’s disease by the acupuncture treatment combined with Danggui Shaoyao San of TCM 【Abstract】 Objective To explore into the probable mechanism of Alzheimer’s disease (AD) by the acupuncture treatment combined with DGSYS of TCM.Methods Three kinds of model rats with dysmnesia which were made by injected into drug of scopolamine or NaNo2 in the abdomen separately and injured at the hippocampal CA1 in brian by drug of quinolinic acid treated by single acupuncture or acupuncture combined with DGSYS of TCM or DGSYS of TCM were observed separately， to compare different influence on dysfunctions of acuquisition and consolidation of memory induced by drugs and injure with nimodipine (NM) or the normal rats.Results The group in which rats treated by acupuncture combined with DGSYS of TCM has better acquisition and consolidation of memory， which is significant difference. The possible mechanism is associated with the regulation of the glutamatergic function、improvement of ability of the cholinerginic system and antioxidation、greatly antagonize the LTP inhibiting effects.Conclusion The acupuncture treatment combined with DGSYS of TCM treating AD in clinic has certain scientific evidences and it is worth being popularized widely. 【Key words】 AD acupuncture/DGSYS/NM scop/NANO2 glutamate/QA AchE/Ach NO/NOS SOD/MDA LTP 为了揭示针刺加当归芍药散治疗阿尔茨海默症（AD）能改善大脑智能发育和精神状态的症状及可能机制［1，2］，我们设计了3种不同实验性痴呆造模方案；进行了探索性研究，不竟获得满意的结果，现将资料整理如下。 1 实验材料 1.1 动物 选用健康5～9月龄灵活性较一致SD雄性大鼠150只，由南京中医药