钠离子通道在异氟醚预处理诱导的脑保护中的作用_医学论文.docVIP

钠离子通道在异氟醚预处理诱导的脑保护中的作用_医学论文 钠离子通道在异氟醚预处理诱导的脑保护中的作用_医学论文 作者：刘艳红，熊利泽，陈绍洋，郑 玉，路志宏，胡 胜 【关键词】 利多卡因 关键词: 利多卡因异氟醚脑保护缺血耐受钠离子通道 摘 要:目的 探讨钠离子通道阻断剂利多卡因在异氟醚预处理所诱导的脑保护中的作用. 方法 40只雄性SD大鼠，随机分为4组（每组n=10），即空白对照组利多卡因对照组，每日给予利多卡因（5mg kg-1 ，ip）连续5d异氟醚处理组，每日给予15mL L-1 异氟醚吸入1h，连续5d利多卡因加异氟醚处理组，在每次异氟醚处理之前给予利多卡因（5mg kg-1 ，ip），余处理同异氟醚处理组.最后一次处理24h后采用颈内动脉尼龙线线栓法致右大脑中动脉栓塞（120min）模型，观察再灌注后24h时神经功能损害并取大脑行TTC染色以测量脑梗死容积. 结果 再灌注24h时神经功能损害评分及脑梗死容积，异氟醚处理组均明显小于其余3组（0.05），而利多卡因加异氟醚处理组及利多卡因处理组的神经功能学评分及脑梗死容积与空白对照组均无明显差异（Pgt0.05）. 结论 本实验进一步证明了多次吸入异氟醚可诱导产生脑保护作用.此作用可被利多卡因阻断，提示了钠离子通道参与异氟醚对脑的预处理效应的形成机制. 　　Keywords:lidocaineisofluraneneuroprotectionischemic tolerancesodium channel 　　Abstract:AIM To investigate the effects of lidocaine，a sodium channel blocker，on the ischemic tolerance induced by　repeated brief isoflurane anesthesia in the focal cerebral is-chemia in rats.METHODS Forty male Sprague-Dawley rats were randomly assigned to four groups（n=10each）:Con-trol Group，animals without treatmentLidocaine Group，animals intraperitoneally injected with lidocaine（5mg kg-1 ，ip.）once a day for5days and Isoflurane Group and Isoflu-rane+Lidocaine Group，animals given15mL L-1 isoflurane1h per day for5days，while those in the Isoflurane+Lido-caine Group received lidocaine（5mg kg-1 ip.）just before each isoflurane pretreatment.24h after the last pretreat-ment，transient MCAO（120min）was induced.The neuro-logical outcome was evaluated24h after the reperfusion.The infarct volume was then assessed with TTC staining after the neurological outcome evaluation.RESULTS The neurologic deficit scores in the Isoflurane Group were lower than those of the other three groups（0.05）.There was no statisti-cal difference between the Control，Isoflurane+Lidocaine and Lidocaine Groups.The infarct volume in the Isoflurane Group was less than those of the other three groups（0.05）.CONCLUSION The present study was demonstrated that repeated isoflurane anesthesia is able to induce ischemic tolerance in transient MCAO rats.This effect can be