香加皮中毒时豚鼠心电图的变化特征_药学论文.docVIP

香加皮中毒时豚鼠心电图的变化特征_药学论文 香加皮中毒时豚鼠心电图的变化特征_药学论文 作者：陈金堂，孙达，毕波，赵春凤，周昆 【摘要】 　　目的寻找香加皮中毒时豚鼠心电图的变化特征，确定是否可以依据豚鼠心电图作为香加皮中毒的判断指标。方法50只豚鼠在清醒状态下连续记录其心电图30 min后，选用心电图正常的豚鼠腹腔注射不同剂量香加皮并记录给药后心电图120 min。对给药后心电异常的豚鼠心电图主要指标进行分析比较。结果给予香加皮后有72%的豚鼠心率减慢。93 mg/kg剂量下豚鼠心电图异常发生率仅为13.04%，剂量186 mg/kg下异常发生率为38.89%，剂量增加到285 mg/kg以上时异常率达到100%，心电图异常发生率与剂量呈正相关。随着香加皮剂量的增大，主要异常依次表现在T波异常、P波和QRS波群异常、QRS波群的脱落。结论豚鼠心电图可以作为香加皮中毒的判断依据。 【关键词】 香加皮；心脏毒性；心电图 　　Abstract：Objective To find the characteristics of guinea pigs’ electrocardiogram changes with Cortex Periplocae poisoning and to determine whether electrocardiogram can be used as a monitoring index in guinea pigs.MethodsThe electrocardiogram of 50 guinea pigs were recorded 30 minutes in a sober state, guinea pigs of normal electrocardiogram were given intraperitoneal injection of different doses of Cortex Periplocae and the electrocardiogram were recorded continuously for 120 minutes after administration. The key indicators of abnormal electrocardiogram were analyzed and compared. ResultsHeart rate of 72% of the guinea pigs that were administered with Cortex Periplocae slowed down. There was positive correlationship between the incidence of abnormal electrocardiogram and dose of Cortex Periplocae, since the abnormal incidence of guinea pigs’ electrocardiogram was 13.04% in 93 mg/kg, 38.89% in 186 mg/kg and 100% above 285 mg/kg. With the dose of Cortex Periplocae increased, the major abnormalities were performanced of the T wave abnormalities, P wave and the QRS wave abnormalities and the QRS wave lost. ConclusionElectrocardiogram can be used as a judgment index with Cortex Periplocae poisoning in guinea pigs. 　　Key words：Cortex Periplocae; Cardiac toxicity; Electrocardiogram 　　香加皮是萝藦科植物杠柳Periploca sepium的干燥根皮，香加皮含有多种强心苷成分，强心苷的治疗指数较低，剂量不易掌握。前期的研究也表明香加皮的毒性及致死性主要体现在心脏毒性，在水合氯醛麻醉的大鼠给药后也观察到了心律失常，心电图有明显的改变，并且有报道用大鼠建立慢性洋地黄中毒模型［1］，但是我们在后续的实验中发现水合氯醛麻醉的大鼠在不给药的情况下也会有少数大鼠发生心律失常，从而导致假阳性结果。笔者转而寻找其它麻醉剂，但经过预试验发现，戊巴比妥钠和乌拉坦麻醉大鼠后，无法用香加皮引起大鼠的心律失常出现，氯醛糖麻醉大鼠后，大鼠肌肉颤动比较严重，心电基线明