柑桔溃疡病拮抗芽孢菌株的筛选及其控病作用.pdfVIP

CHINA FRUITS 中国南方果树／SOUTH 2012；41(4)：5～7，17 柑桔溃疡病拮抗芽孢菌株的筛选及其控病作用 易 龙1，马冠华2 (1赣南师范学院生命与环境科学学院，赣州，341000；2西南大学植物保护学院) 摘要：从赣州脐橙园脐橙根际土壤中获得192个细菌分离物，通过平板拮抗测试，筛选出对柑桔 溃疡病菌Xanthomonasaxonopodispv．citri有较强拮抗作用的6个芽孢菌株，抑茵圈直径达12．0 ～25．0 ITIITI，其中菌株G32对柑桔溃疡病茵抑制效果最好，稳定性高；温室控病试验表明，菌株G32 的控病效果最佳，接种溃疡病菌前喷施拮抗菌G32的预防效果达60．7％，接种溃疡病菌后喷施拮 rDNA序列比对 抗茵G32的治疗效果为53．2％；根据其形态特征、培养性状、生理生化特性及16S 分析，将G32鉴定为枯草芽孢杆茵Bacillussubtilis。 关键词：柑桔溃疡病；枯草芽孢杆菌；生物防治 中图分类号：S436．661．1+9；S of BacteriaforBiocontrol ScreeningAntagonisticSpore against Xanthomonas axonopodispv．citri Yi Guanhua2 Lon91。Ma ofLifeandEnvironmental Normal ofPlant (1College Sciences，GannanUniversity，Ganzhou，341000；2Coliege Protection，SouthwestUniversity) isolateswereobtainedfrom soilofcitrusorchardsin Abstract：192 rhizosphere Ganzhou，Jiangxi was foundthat6 biocontrolbacteriashowed effecton province．It potential spore goodantagonistic Xanthornonas ofcitrus bacterialcanker，for inhibition axonopodispv．citri，thepathogen shoⅥd119 ITinl zonesof12．0 to25．0rmindiameter cultureonNA bacterialstrainG32 bypairing plate．The hadthebest effectonthe andthe controlrateofstrain inhibitory pathogen higheststability．The G32reached60．7％and when toandaftertheinoculationofcitrus 53．2％respectivelysprayedprior cankerbact