去卵巢大鼠海马及额叶皮层海马胆碱能神经刺激肽含量的变化_临床医学论文.docVIP

去卵巢大鼠海马及额叶皮层海马胆碱能神经刺激肽含量的变化_临床医学论文 去卵巢大鼠海马及额叶皮层海马胆碱能神经刺激肽含量的变化_临床医学论文 作者：高飞 宋士军 胡进中 李芳芳 张若楠 【摘要】 目的 观察雌激素缺乏对大鼠海马CA1/CA2区及额叶皮层海马胆碱能神经刺激肽（HCNP）含量的影响。方法 选取清洁级4月龄雌性SD大鼠30只，随机分为3组：假手术组（Sham组）、去卵巢组（OVX组）和去卵巢加雌二醇（E2）组（OVX+E2组），每组10只。除Sham组做假性手术外，其余2组均行双侧卵巢切除术；OVX+E2组去卵巢手术10 d后给予E2皮下注射，100 μg/kg，隔日1次。去卵巢手术后常规喂养4个月，获取组织标本，用于组织蛋白和组织总RNA的提取。用Western印迹法测定大鼠海马和皮层C、N的相对含量。用RT技术检测HCNPpp mRNA水平。结果 OVX组大鼠海马和皮层中C的相对含量较Sham组和OVX+E2组明显升高(P＜0.05)，而Sham组和OVX+E2组之间无显著差别(P＞0.05)，三组大鼠之间的海马和皮层中N的相对含量差异不显著(P＜0.05)；OVX组和OVX+E2组大鼠HCNPpp mRNA表达与Sham组无显著差异(P＞0.05)，OVX+E2组大鼠HCNPpp mRNA表达与OVX组相比增高(P＜0.05)；三组大鼠皮层HCNPpp mRNA表达无显著差异(P＞0.05)。结论 雌激素缺乏可导致大鼠海马CA1/CA2区及额叶皮层HCNP的增多。 【关键词】 去卵巢；雌激素；海马；额叶皮层；海马胆碱能神经刺激肽（HCNP） 【Abstract】 Objective To observe the effect of the defect of estrogen on the contents of HCNP in regions of CA1/CA2 of hippocampus and the expression of HCNPpp mRNA in prefrontal cortex of ovariectomized（OVX）SD rats. Methods Thirty female clean SD rats aged 4 months were randomly divided into sham, OVX, OVX and estradiol (OVX+E) groups, and ten rats per group. Sham group was ablated some adipose tissue above ovaries, OVX group was ablated two sides ovaries, OVX+E group was supplied by estradiol (100 μg/kg, qod) after 10 d of ovariectomy. Postoperative rats were fed by routine method and then sacrificed 4 months later to obtain tissues and specimen for the use of the extraction of tissues’ protein and total RNA.The relative protein contents of CHCNPpp and NHCNPpp were measured by Western blotting, and the expression of HCNPpp mRNA was measured by RTPCR in the dorsal hippocampus and prefrontal cortex of ovariectomized rats. Results Compared with that of sham group and OVX+E group,the relative content of CHCNPpp of OVX group in hippocampus and prefrontal cortex was higher, and there was not different between sham group and OVX+E group (Pgt;0.05). However，the expression of NVX group and OVX+E group in hippocampus was not different(Pgt;0.05).Compared with that of OVX group, the relative exp