参麦注射液对急性心肌梗死大鼠心室重构的影响_临床医学论文.docVIP

参麦注射液对急性心肌梗死大鼠心室重构的影响_临床医学论文 参麦注射液对急性心肌梗死大鼠心室重构的影响_临床医学论文 作者：温晓竞 陈彦静 李建东 马建伟 张晓云 【摘要】 目的：探讨参麦注射液对急性心肌梗死（AMI）大鼠心室重构的影响。方法：通过结扎冠状动脉前降支建立AMI模型大鼠，AMI后24h存活的48只大鼠随机分为四组：AMI后1周模型组；AMI后参麦注射液治疗1周组；AMI后2周模型组；AMI后参麦注射液治疗2周组。另设1周、2周假手术组各8只。治疗组每日腹腔注射参麦注射液1次，AMI模型组和假手术组大鼠腹腔注射等量的生理盐水。测量各组心室重构参数。结果：与假手术组比较，AMI后1周时，心室腔已明显扩大（005）；到梗死后2周时，心室腔内径扩大更明显，与假手术组相比有显著性差异（001），且心室重量指数（VWI）比假手术组明显增加（005）。参麦注射液治疗2周后可改善心梗后心室重量指数增加、心室腔扩大的病理变化（005）。结论：参麦注射液能改善大鼠AMI后心室重构。 【关键词】 参麦注射液；急性心肌梗死；心室重构 【ABSTRACT】 Objective: To investigate the effects of Shenmai injection on ventricular remodeling after acute myocardial infarction(AMI) in rats. Methods: AMI was established by ligating the anterior descending coronary artery in male SD rats. Twentyfour hours after the procedure, the 48 surviving rats were seperated randomly into AMI one week group、 AMI twoweek group、Shenmai injection treatment oneweek and twoweek groups (n=12). Oneweek shamoperated and twoweek shamoperated groups were taken randomly as noninfarction control. After treated with the drug and placebo by intraperitoneal injection(IP), the ventricular remodeling of oneweek and twoweek groups was assessed. Results: Compared with shamoperated group, left ventricular diameter(LVD) was increased significantly in AMI group after a week（005）；LVD and ventricular weight index(VWI) were all increased significantly（005 or 001）in AMI group after two weeks. In comparison with AMI group, LVD and VWI were all decreased significantly in Shenmai injection treatment group after two weeks（005）. Conclusion: Shenmai injection can prevent left ventricular remodeling after AMI in rats. 【KEY WORDS】 Shenmai injection; Acute myocardial infarction; Ventricular remodeling 参麦注射液源自古方生脉散，具有益气复脉、养阴生津的功效，临床上广泛用于治疗心脑血管疾病。近年来的研究发现，参麦注射液具有扩张冠状动脉，增加冠脉血流量，促进损伤的DNA合成，加速心肌修复的作用，还可以改善心肌缺氧，增加心肌收缩力。但关于参麦注射液对急性心梗后心室重构作用的深入研究较少，尤其对心梗后早期重构的干预研究不足。因此本实验着重研究参麦注射液对心梗后早期左心室重构发生、发展的影响特点，探讨参麦注射液改善心梗后心室重构的机制，为其更广泛深入的临床应用提供科学的实验依据。 1 材料和方法 11 材料 111 实验动物健康雄性10～12周龄SD大鼠70只，体重250～280g，由中国科学院