吉非替尼对结肠癌细胞的生长抑制作用与PTEN表达的关系_临床医学论文.docVIP

吉非替尼对结肠癌细胞的生长抑制作用与PTEN表达的关系_临床医学论文 吉非替尼对结肠癌细胞的生长抑制作用与PTEN表达的关系_临床医学论文 作者：杨黎 潘凤 陈玉英 梁后杰【摘要】 目的 观察表皮生长因子受体酪氨酸激酶抑制剂吉非替尼对人结肠癌细胞的生长抑制作用，探讨这种作用与结肠癌细胞PTEN表达的关系。方法 应用体外药物敏感实验检测吉非替尼对6种人结肠癌细胞系的生长抑制作用；应用RT-PCR检测不同结肠癌细胞中PTEN mRNA水平；应用Western blot检测结肠癌细胞中PTEN蛋白表达水平。结果 吉非替尼在体外对6种结肠癌细胞系的生长抑制作用差异很大（F=325.51，0.05）。吉非替尼作用浓度为1 μmol/L时，Lovo细胞系抑制率达34%，对吉非替尼最为敏感， 其半量抑制浓度（IC50）lt;10 μmol/L； HT29和SW480为中度敏感（10 μmol/L100 μmol/L。各个细胞系中PTEN mRNA和PTEN蛋白均有表达。结论 吉非替尼对结肠癌细胞的生长抑制作用与PTEN mRNA和PTEN蛋白表达水平无明显相关，即PTEN表达状态还不能作为预测结肠癌对吉非替尼敏感性的可靠生物学指标。 【关键词】 结肠肿瘤； 细胞系； PTEN； 吉非替尼； 生长抑制 　　Relationship between PTEN expressions and inhibitory effects of Gefitinib on the growth of colon cancer cells 【Abstract】 Objective To observe the inhibitory effect of Gefitinib, a selective oral epidermal growth factor receptor tyrosine kinase inhibitor, on the growth of human colon cancer cells, and investigate the relationship between the sensitivity of colon cancer cells to Gefitinib and PTEN expressions. Methods The inhibitory effects of Gefitinib on 6 kinds of colon cancer cells （Lovo, HCT116, HT29, LS174T, SW480 and SW620）, the levels of PTEN mRNA in different colon cancer cells, and PTEN protein expressions in colon cancer cells were detected by MTT assay, RT-PCR and Western blot, respectively. Results The inhibitory effects of Gefitinib on the 6 colon cancer cells in vitro varied a lot. When the concentration of Gefitinib was 1 μmol/L, Lovo cells had the most sensitivity to Gefitinib with an IC50lt;10 μmol/L; HT29 and SW480 had moderate sensitivity, and the IC50 ranged from 10 μmol/L to 100 μmol/L; HCT116, LS174T and SW620 were insensitive to Gefitinib, and their IC50gt;100 μmol/L. All the colon cancer cell lines exhibited PTEN mRNA and protein expressions. Conclusions PTEN mRNA and protein expressions might not be associated with the inhibitory effects of Gefitinib on the growth of colon cancer cells. The expression of PTEN can not be taken as the indication of the sensitivity of colon cancer cells to Gefitinib.