同种异体间充质干细胞复合纤维蛋白修复骨缺损_临床医学论文.docVIP

同种异体间充质干细胞复合纤维蛋白修复骨缺损_临床医学论文 同种异体间充质干细胞复合纤维蛋白修复骨缺损_临床医学论文 作者：窦强兵，孔荣，朱六龙，戴永立，沈勇 【关键词】 松质骨缺损 　　摘 要： 　　［目的］探讨骨髓间充质干细胞(mesenchymal stem cells，MSCs)的体外培养，以及同种异体MSCs复合纤维蛋白修复兔股骨髁松质骨缺损的效果。［方法］在日本大耳兔双侧股骨髁制作06 cm×10 cm松质骨缺损，左侧植入同种异体MSCs纤维蛋白复合物，右侧单纯植入纤维蛋白。分别于术后2、5、8周行放射学、组织学检查，以了解骨缺损修复情况。［结果］单纯植入纤维蛋白的一侧不能自行愈合。植入复合物的一侧术后2周可见少量骨组织生成，5周可见大量模糊骨痂，术后8周骨缺损基本愈合。除少量炎性细胞浸润外，各期均未见明显免疫排斥反应。［结论］同种异体MSCs复合纤维蛋白可有效修复兔股骨髁松质骨缺损，8周内机体的免疫排斥反应较弱。 　　关键词：松质骨缺损； 间充质干细胞； 纤维蛋白； 同种异体 　　Abstract：［Objective］To discuss bone marrow derived mesenchymal stem cells (MSCs) culture in vitro and the curative effect of repairing cancellous bone defects in rabbits with the compound of allogenic MSCs and fibrin. ［Method］MSCs were isolated and cultureexpanted from rabbit bone marrow. Cancellous bone defects were created on bilateral femur condylar in 15 Japanese big ear rabbits, the left bone defects were treated with allogenic MSCs and fibrin, the right were treated with fibrin only. The bone defects were examined radiologically and histoloyically at the 2nd, 5th, 8th week after operation. ［Result］The defects with only fibrin implantation couldnt heal spontaneously and no bone formation were found at any timepoint. In the defects with compound of MSCs and fibrin implantation, there were a few bone formation within 2 weeks. By the 5th week plentiful callus were found, at the end of the 8th week, the defects healed. There was no immune respone except a few inflammatory cells infiltrate. ［Conclusion］The compound grafts of allogenic MSCs and fibrin shows low immunogenicity within 8 weeks and is an effective method for repairing cancellous bone defects. 　　Key words: Cancellous bone defect; Mesenchymal stem cells (MSCs); Fibrin; Allogenic 　　骨缺损常发生于创伤、感染、肿瘤切除等疾病，是骨科常见难题，在治疗上近年兴起的以间充质干细胞(mesenchymal stem cells, MSCs)为种子细胞的组织工程骨修复具有广阔的临床应用前景。本实验采用同种异体MSCs与纤维蛋白复合物修复兔股骨髁骨缺损，观察异体MSCs在体内的成骨及免疫情况，为临床使用同种异体MSCs提供实验依据。 　　1 材料和方法 　　11 MSCs的分离培养 　　4个月龄青紫蓝兔1只，重20 kg，用含肝素30 u／ml的注射器于股骨粗隆抽取骨髓2 ml， 加入含10％胎牛血清和青霉素及链霉素的DMEM培养基(Gibsco公司)中吹打