喉癌患者血清可溶性白细胞介素_临床医学论文.docVIP

喉癌患者血清可溶性白细胞介素_临床医学论文 喉癌患者血清可溶性白细胞介素_临床医学论文 作者：姚俊，陈仁辉，王军，崔德威 【关键词】 喉癌 摘 要：目的：研究血清可溶性白细胞介素-受体（sIL-R）和一氧化氮（NO）水平在喉癌患者中的检测和临床意义。方法：应用单克隆、多克隆双抗体夹心ELISA及硝酸还原酶法检测sIL-R和NO水平。40例喉癌患者为观察组，同期住院的29例声带息肉患者作为良性对照组，30名健康志愿者作为健康对照组。结果：喉癌患者sIL-R水平较良性对照组或健康对照组显著升高（0.001），与喉癌淋巴结转移、临床分型亦密切相关；喉癌患者NO水平显著高于良性对照组或健康对照组（0.01）。结论：血清sIL-R 水平检测可做为喉癌诊断的一项辅助指标，并有助于了解喉癌的大小，病程发展及淋巴结转移情况等；NO可能在分子水平上参与了喉癌的发生发展，其检测可作为喉癌患者病情演变的监测指标。 　　关键词：喉癌； 血清可溶性白细胞介素-受体；一氧化氮 　　The Detection and Significance of sIL-R and NO in Serum of Patients with Laryngeal Cancer 　　　Abstract: Objective： To research the detection and clinical significance of sIL-R and NO in serum of patients with laryngeal cancer. Method： Detected the level of sIL-R and NO in serum through monoclonal and polyclonal diploantibody enzyme-linked immunoadsordent assay and nitrate reductase methods. There were 40 patients with laryngeal cancer in observing group， and 29 cases of vocal polyp of the same hospital in benign constracting group. 30 cases healthy volunteer were treated as healthy constracting group. Result： The level of sIL-R in patients with laryngeal cancer was significantly higher than benign contrasting group or healthy volunteer group (0.001)， and it was closely related to the condition of lymphonodus metastasis and clinical typing. The level of NO in observing group was significantly higher than benign contrasting group or healthy volunteer group (0.01).Conclusion： The detection of sIL-R in serum can be applied as an assist marker of diagnosing laryngeal cancer. It will be useful to know the size ， course and the condition of lymphonodus metastasis of the cancer . NO may be take part in the genesis and development of laryngeal cancer from molecule level and its detection is a useful parameter to estimate the development of laryngeal of cancer . 　　Key words: Laryngeal cancer； sIL-R； NO 　　可溶性白细胞介素-受体（sIL-R）现今已被作为某些恶性肿瘤的诊断、预后与指导治疗的一项重要指标，而一氧化氮（NO）是一种新型的生物细胞信使分子和效应分子，对肿瘤的增长、转移有一定的作用及影响［1］。但目前有关可溶性白细胞介素-受体（sIL-R）和一氧化氮（NO）水平在喉癌中变化情况的报道极少，