喘息康对支气管哮喘大鼠外周血浆IFNγ，IL5，Eos和Eotaxin的影响_临床医学论文.docVIP

喘息康对支气管哮喘大鼠外周血浆IFN，IL，Eos和Eotaxin的影响_临床医学论文 喘息康对支气管哮喘大鼠外周血浆IFN，IL，Eos和Eotaxin的影响_临床医学论文 【摘要】 目的： 研究喘息康对哮喘大鼠模型外周血清中干扰素白细胞介素嗜酸性粒细胞(Eos)及嗜酸细胞活化趋化因子(Eotaxin)的影响，探讨其治疗支气管哮喘的作用机制. 方法： 将40只大鼠随机分组为正常对照组,哮喘模型组,喘息康中药组,地塞米松西药组，每组10只. 采用卵白蛋白(OVA)腹腔注射致敏和雾化激发的方法建立大鼠支气管哮喘模型，然后以不同的方法对哮喘大鼠模型进行干预. 采用ELISA方法检测每只大鼠血清中IFN及Eotaxin水平，取血清进行Eos计数. 结果： 哮喘模型组与正常对照组相比，血清中IFN水平降低(0.01),而IL和Eos计数则升高(0.01),并且引喘潜伏期缩短(0.05)；喘息康中药组和地塞米松西药组与哮喘模型组相比，血清中IFN水平高于哮喘模型组(0.05),而IL和Eos计数则降低(0.05),并且引喘潜伏期延长(0.05). 结论： 喘息康可以通过促进哮喘大鼠体内干扰素分泌和抑制IL表达，而降低支气管高反应性（BHR）；并可通过抑制Eotaxin的表达而减少嗜酸性粒细胞(EOS)在肺部的聚集和活化,从而减轻气道EOS浸润,使引喘潜伏期延长,从而达到治疗哮喘的目的. 【关键词】 喘息康；哮喘；干扰素Ⅱ型；白细胞介素；嗜酸细胞；嗜酸细胞活化趋化因子　　【Abstract】 AIM： To investigate the effects of Chuanxikang decoction(a Chinese herbal medicine)on peripheral plasma levels of IFN： A total of 40 rats were randomly divided into 4 groups： normal control group, asthmatic model group, Chuanxikang decoction group, and dexamethasone group, with 10 rats in each group. The rat models of asthma were established through injecting ovalbumin(OVA) into the abdominal cavity and inhalation of atomization. The animals underwent different interventions. The effects of Chuanxikang decoction on plasma levels of IFNγ, IL5 and Eotaxin were detected by immunohistochemical technology and the number of Eos was also determined. RESULTS： Compared with the normal control group, the concentrations of IFNwas decreased significantly (0.01) and IL5, Eotaxin and the number of Eos were increased significantly (0.01) in the asthmatic model group, and asthmainduced delitescence was also shortened obviously(0.05). Compared with the asthmatic model group, IFNγ in the serum of the dexamethasone group and Chuanxikang decoction group was increased significantly(0.05), while IL, Eotaxin and the number of Eos of them were decreased significantly(0.05), and asthmainduced delitescence was prolonged obviously(0.05). CONCLUSION: Chuanxikang has therapeutic effect on bronchial asthma by increasing the level of IFNγ and inhibit