基质金属蛋白酶-9在大肠癌中的表达及其与血管生成的相关性_临床医学论文.docVIP

基质金属蛋白酶-9在大肠癌中的表达及其与血管生成的相关性_临床医学论文 基质金属蛋白酶-9在大肠癌中的表达及其与血管生成的相关性_临床医学论文 【摘要】 　　［目的］ 探讨基质金属蛋白酶-9(MMP-9)在大肠癌中的表达及其与血管生成关系的临床意义。［方法］ 应用免疫组化SP法检测108例大肠癌组织中MMP-9、血管内皮生长因子(VEGF)的表达以及微血管密度(MVD)。［结果］ MMP-9、VEGF的表达和MVD值与肿瘤分化、淋巴结转移、Dukes分期明显相关；MMP-9、VEGF阳性表达组的MVD值显著高于阴性组；MMP-9与VEGF在大肠癌组织中表达呈正相关。［结论］ MMP-9与大肠癌血管生成有关，检测MMP-9的表达可作为反映大肠癌侵袭和转移的生物学指标。 【关键词】 大肠肿瘤　基质金属蛋白酶-9　血管内皮生长因子　微血管密度　血管生成　免疫组织化学 　　Expression of Matrix Metalloproteinases-9 and Its Relationship with Angiogenesis in Colorectal Carcinoma Abstract：［Purpose］ To study the clinical significance and the relationship between the expression of matrix metalloproteinases-9 (MMP-9) and angiogenesis in colorectal carcinoma. ［Methods］ The expressions of MMP-9, vascular endothelial growth factor (VEGF) and microvessel density (MVD) in 108 cases with colorectal cancer were detected by S-P immunohistochemical method. ［Results］ The expressions of MMP-9 and VEGF were significantly correlated with the numbers of MVD and the differentiation, lymph node metastasis and Dukes stage in colorectal cancer. The value of MVD in cases with the positive expressions of MMP-9 and VEGF was higher than that in cases with the negative ones. There was a positive correlation between expressions of MMP-9 and VEGF in colorectal carcinoma. ［Conclusion］ There is a close relationship between the expression of MMP-9 and angiogenesis in colorectal carcinoma. The expression of MMP-9 can be used as a biological marker for cancer agreesiveness and metastasis in colorectal carcinoma. Key words: colorectal neoplasms; matrix metalloproteinase-9; vascular endothelial growth factor; microvessel density; angiogenesis; immunohistochemistry 大肠癌预后不良的主要原因为浸润转移和术后易复发，其浸润转移过程复杂，而血管生成起着重要的作用。血管生成不仅为肿瘤细胞增殖提供养分，而且是肿瘤细胞进一步侵袭的重要途径。基质金属蛋白酶（matrix metalloproteinases，MMPs）通过对细胞外基质（extracelluar matrix，ECM）和血管基底膜的降解，在许多恶性肿瘤血管生成中发挥重要作用[1]。我们通过免疫组化SP法检测大肠癌组织中基质金属蛋白酶9（matrix metalloproteinases-9，MMP-9）、血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达以及微血管密度(microvessel density,