壮元扶骨逍遥汤治疗膝骨关节炎的实验研究_临床医学论文.docVIP

壮元扶骨逍遥汤治疗膝骨关节炎的实验研究_临床医学论文 壮元扶骨逍遥汤治疗膝骨关节炎的实验研究_临床医学论文 作者：马永健 李青 王金榜 张丽娜【摘要】 　　目的 观察壮元扶骨逍遥汤对家兔膝骨关节炎模型血清中超氧化物歧化酶（SOD）和关节液中白介素-1（IL-1）含量的影响，并探讨其作用机制。方法 将新西兰白兔36只，雌雄不分，随机分成4组，即正常组（N组）、模型对照组（M组）、壮元扶骨逍遥汤组（F组）、布洛芬组（P组），各8只，于相同温度、湿度下分别单笼喂养，在治疗前、中、后分别提取家兔的血清和关节液，采用免疫组织化学法检测血清SOD和关节液IL-1的含量。结果 治疗前比较，M、F、P组SOD水平明显降低（P＜0.05），IL-1水平明显升高（P＜0.05）；治疗中、后期比较，F组SOD水平明显升高（0.05），P组无明显变化（Pgt;0.05），F、P组IL-1水平明显降低（0.05）。结论 壮元扶骨逍遥汤可提高血清SOD水平，通过激活SOD的活性而使其含量显著升高，催化氧自由基发生歧化反应，阻止过多氧自由基对关节软骨的损害，保护关节软骨，提高软骨细胞功能，促进软骨修复；降低关节液IL-1水平，可有效清除促炎性细胞因子，减轻炎症对关节软骨的破坏及所产生的疼痛，可能是通过抑制巨噬细胞的分泌来起效的。 【关键词】 骨关节炎；膝；动物；实验；壮元扶骨逍遥汤 　　Experimental investigation on treating osteoarthritis of the knee with Zhuangyuanfuguxiaoyao decoction 　　【Abstract】 Objective To observe the influence of Zhuangyuanfuguxiaoyao decoction on SOD in blood serum and IL-1 in synovial fluid of knee osteoarthritis rabbit model, and reveal the mechanism of action. Methods 36 well-being New Zealand white rabbits were randomly divided into 4 group, they are normal group(group N), model reference group(group M), Zhuangyuanfuguxiaoyao decoction group(group Z), ibuprofen group(group P), 8 rabbits in each group. The rabbits blood serum and synovial fluid were extracted in pre-treatment, meta- treatment and post- treatment, and SOD and IL-1 levels were detect with the immunohistochemistry. Result The concentration of SOD in group M, group Z and group P before treatment could markedly reduce compared with that in group N (0.05), and the concentration of IL-1 increased (0.05). The concentration of SOD meta- treatment and post- treatment group Z was higher than those pre-treatment (0.05), but group P there was no significant difference (Pgt;0.05). The concentration of IL-1 meta- treatment and post- treatment group Z and group P were lower than those pre-treatment (Pgt;0.05). Conclusion Zhuangyuanfuguxiaoyao decoction can enhance the level of blood serum SOD by activating activity of SOD, and prevent damage of arthrodial cartilage from oxygen radical by c